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A Y-linked anti-Müllerian hormone type-II receptor is the sex-determining gene in ayu, Plecoglossus altivelis
https://oacis.repo.nii.ac.jp/records/2332
https://oacis.repo.nii.ac.jp/records/2332372ce231-adad-419c-a656-323e9177bf01
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-03-23 | |||||
タイトル | ||||||
タイトル | A Y-linked anti-Müllerian hormone type-II receptor is the sex-determining gene in ayu, Plecoglossus altivelis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Nakamoto, Masatoshi
× Nakamoto, Masatoshi× Uchino, Tsubasa× Koshimizu, Eriko× Kuchiishi, Yudai× Sekiguchi, Ryota× Liu, Wang× Sudo, Ryusuke× Endo, Masato× Guiguen, Yann× Schartl, Manfred× John H. Postlethwait× Sakamoto, Takashi |
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書誌情報 |
PLOS Genetics 発行日 2021-08-26 |
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抄録 | ||||||
内容記述 | Whole-genome duplication and genome compaction are thought to have played important roles in teleost fish evolution. Ayu (or sweetfish), Plecoglossus altivelis, belongs to the superorder Stomiati, order Osmeriformes. Stomiati is phylogenetically classified as sister taxa of Neoteleostei. Thus, ayu holds an important position in the fish tree of life. Although ayu is economically important for the food industry and recreational fishing in Japan, few genomic resources are available for this species. To address this problem, we produced a draft genome sequence of ayu by whole-genome shotgun sequencing and constructed linkage maps using a genotyping-by-sequencing approach. Syntenic analyses of ayu and other teleost fish provided information about chromosomal rearrangements during the divergence of Stomiati, Protacanthopterygii and Neoteleostei. The size of the ayu genome indicates that genome compaction occurred after the divergence of the family Osmeridae. Ayu has an XX/XY sex-determination system for which we identified sex-associated loci by a genome-wide association study by genotyping-by-sequencing and whole-genome resequencing using wild populations. Genome-wide association mapping using wild ayu populations revealed three sex-linked scaffolds (total, 2.03 Mb). Comparison of whole-genome resequencing mapping coverage between males and females identified male-specific regions in sex-linked scaffolds. A duplicate copy of the anti-Müllerian hormone type-II receptor gene (amhr2bY) was found within these male-specific regions, distinct from the autosomal copy of amhr2. Expression of the Y-linked amhr2 gene was male-specific in sox9b-positive somatic cells surrounding germ cells in undifferentiated gonads, whereas autosomal amhr2 transcripts were detected in somatic cells in sexually undifferentiated gonads of both genetic males and females. Loss-of-function mutation for amhr2bY induced male to female sex reversal. Taken together with the known role of Amh and Amhr2 in sex differentiation, these results indicate that the paralog of amhr2 on the ayu Y chromosome determines genetic sex, and the male-specific amh-amhr2 pathway is critical for testicular differentiation in ayu. | |||||
内容記述 | ||||||
内容記述 | この研究のプレスリリース版はこちら: http://id.nii.ac.jp/1342/00002272/ | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12073492 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1371/journal.pgen.1009705 | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1371/journal.pgen.1009705 | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | http://id.nii.ac.jp/1342/00002272/ | |||||
関連名称 | Press Release | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K14972/ | |||||
関連名称 | KAKEN研究課題: 次世代シーケンサーを用いた天然魚における優良形質の全ゲノム相関解析 | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20H00431/ | |||||
関連名称 | KAKEN研究課題: 魚類における耐病性責任遺伝子の同定と新規疾病が野生集団に与える遺伝的影響の解析 | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000040313390/ | |||||
関連名称 | 研究代表者: 坂本 崇 (東京海洋大学) | |||||
情報源 | ||||||
関連識別子 | https://plos.org/ | |||||
関連名称 | PLOS | |||||
出版者 | ||||||
出版者 | PLOS | |||||
科学研究費研究課題 | ||||||
主題 | 次世代シーケンサーを用いた天然魚における優良形質の全ゲノム相関解析 | |||||
科学研究費研究課題 | ||||||
主題 | Genome wide association study of the useful traits in wild fish using the next generation sequencer | |||||
科学研究費研究課題 | ||||||
主題 | 魚類における耐病性責任遺伝子の同定と新規疾病が野生集団に与える遺伝的影響の解析 | |||||
研究課題番号 | ||||||
内容記述 | 16K14972 | |||||
研究課題番号 | ||||||
内容記述 | 20H00431 |