| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-09-09 |
| タイトル |
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|
タイトル |
Custom-made medium approach for effective enrichment and isolation of chemolithotrophic iron-oxidizing bacteria |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
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主題 |
custom-made medium, enrichment, Gallionellaceae, iron-oxidizing bacteria, isolation |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
| 著者 |
Uchijima, Tomoki
Kato, Shingo
Tanimoto, Kazuya
Shiraishi, Fumito
Hamamura, Natsuko
Tokunaga, Kohei
Makita, Hiroko
Kondo, Momoko
Ohkuma, Moriya
Mitsunobu, Satoshi
|
| 書誌情報 |
en : FEMS Microbiology Ecology
巻 101,
号 6,
p. fiaf051,
発行日 2025-06-01
|
| 抄録 |
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内容記述 |
Chemolithotrophic neutrophilic iron (Fe)-oxidizing bacteria, which mainly belong to the family Gallionellaceae, universally prevail in terrestrial environments changing Fe cycling. However, they are typically recognized as difficult-to-culture microbes. Despite efforts, there are few Fe(II)-oxidizing lithotroph isolates; hence, their physiological and ecological knowledge remains limited. This limitation is largely owing to difficulties in their cultivation, and we hypothesize that the difficulty exists because substrate and mineral concentrations in the cultivation medium are not tuned to a specific environmental condition under which these organisms live. To address this hypothesis, this study proposes a novel custom-made medium approach for chemolithotrophic Fe(II)-oxidizing bacteria; a method that manipulates medium components through diligent analysis of field environment. A new custom-made medium simulating energy substrates and nutrients under the field condition was prepared by modifying both chemical composition and physical setup in the glass-tube medium. In particular, the modification of the physical setup in the tube had a significant effect on adjusting dissolved Fe(II) and O2 concentrations to the field environment. Using the medium, Gallionellaceae members were successfully enriched and a new Gallionellaceae species was isolated from a natural hot spring site. Compared with conventional medium, the custom-made medium has significantly higher ability in enriching Gallionellaceae members. |
| 内容記述 |
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内容記述 |
公開日: 2025-05-05 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0168-6496 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10517278 |
| DOI |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/femsec/fiaf051 |
| 情報源 |
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|
関連識別子 |
https://academic.oup.com/femsec/article/101/6/fiaf051/8126031 |
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|
関連名称 |
Publisher's Version/PDF (Open Access) |
| 情報源 |
|
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|
関連識別子 |
https://academic.oup.com/femsec/article/101/6/fiaf051/8126031 |
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|
関連名称 |
Oxford University Press |
| 出版者 |
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出版者 |
Oxford University Press |
| 科学研究費研究課題 |
|
|
言語 |
ja |
|
主題URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19H03087/ |
|
主題 |
最先端X線分光法を駆使した水田土壌表層へのヒ素濃集機構の解明と土壌修復への応用 |
| 科学研究費研究課題 |
|
|
言語 |
en |
|
主題URI |
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19H03087/ |
|
主題 |
Study on arsenic accumulation mechanism in surface layer of paddy soils using advanced X-ray spectroscopy and its application to soil remediation technique |
| 科学研究費研究課題 |
|
|
言語 |
ja |
|
主題URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19H04302/ |
|
主題 |
鉱山廃棄物の持続的な安定化に向けた有害金属の微生物ー鉱物相互作用の解明 |
| 科学研究費研究課題 |
|
|
言語 |
en |
|
主題URI |
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19H04302/ |
|
主題 |
Microbial-mineral interactions of toxic metals for stabilization of mine waste |
| 科学研究費研究課題 |
|
|
言語 |
ja |
|
主題URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-21K19871/ |
|
主題 |
微生物電気化学プロセスを活用した持続可能な汚染浄化と新規ナノ材料創生技術の開発 |
| 科学研究費研究課題 |
|
|
言語 |
ja |
|
主題URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23K27042/ |
|
主題 |
水田土壌表層へのヒ素異常濃集を支配する複合要因の解明と自然模倣型土壌浄化への応用 |
| 科学研究費研究課題 |
|
|
言語 |
en |
|
主題URI |
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23K27042/ |
|
主題 |
Investigation of complex factors controlling arsenic accumulation in surface paddy soils and its application to nature-mimetic soil remediation |
| 科学研究費研究課題 |
|
|
言語 |
ja |
|
主題URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-23H00534/ |
|
主題 |
SDGsバイオ技術開発：微生物電気化学プロセスによる持続的な汚染浄化と金属回収 |
| 科学研究費研究課題 |
|
|
言語 |
en |
|
主題URI |
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23H00534/ |
|
主題 |
SDGs Biotechnology Development: Sustainable Pollution Remediation and Metal Recovery through Microbial Electrification Processes |
| 研究課題番号 |
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|
内容記述 |
19H03087 |
| 研究課題番号 |
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|
内容記述 |
19H04302 |
| 研究課題番号 |
|
|
内容記述 |
21K19871 |
| 研究課題番号 |
|
|
内容記述 |
23K27042 |
| 研究課題番号 |
|
|
内容記述 |
23H00534 |
