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Identification of a rabbit Ig light chain recombinant protein bound to serum immunoglobulins from different marine fish species
https://oacis.repo.nii.ac.jp/records/2571
https://oacis.repo.nii.ac.jp/records/25716a931c2d-b2a0-441d-9148-f041a8782de1
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-11-21 | |||||
タイトル | ||||||
タイトル | Identification of a rabbit Ig light chain recombinant protein bound to serum immunoglobulins from different marine fish species | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Phage display, Library, Immunoglobulin, Antibody | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Jirapongpairoj, Walissara
× Jirapongpairoj, Walissara× Nozaki, Reiko× Koiwai, Keiichiro× Hirono, Ikuo× Kondo, Hidehiro |
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書誌情報 |
Fish & Shellfish Immunology 巻 127, p. 939-947, 発行日 2022-08 |
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抄録 | ||||||
内容記述 | The structures of fish serum immunoglobulin differ among different fish species. In this study, we accidently isolated a rabbit immunoglobulin (Ig) light chain bound to serum immunoglobulin from different marine fish species using phage display. Fish Ig was separated using a protein A column. The phage library was generated from variable regions of rabbit spleen B cells immunized with bluefin tuna Thunnus orientalis Ig. Fish Ig–specific phages were enriched using two rounds of bio-panning with yellowtail Seriola quinqueradiata serum Ig, followed by two rounds of bio-panning with red seabream Pagrus major serum Ig. The enriched phages demonstrated an increase in binding specificity to the tuna, yellowtail, and red seabream Igs compared to the phages listed in the unpanned library. A recombinant protein of a single clonal phage, which encodes the rabbit Ig light chain, was produced, and the binding specificities to fish Igs were analyzed using enzyme-linked immunosorbent assay (ELISA) and western blotting. The recombinant protein exhibited binding properties to fish Igs in the ELISA. However, the recombinant protein that bound to serum protein(s), but not IgM, was detected via western blotting. The recombinant protein may provide a novel information on the common structural feature in the fish immunoglobulins. |
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内容記述 | ||||||
内容記述 | 公開日: 2023-07-21 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1050-4648 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10838941 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 35868474 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.fsi.2022.07.032 | |||||
権利 | ||||||
権利情報 | (c) 2022 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.fsi.2022.07.032 | |||||
情報源 | ||||||
関連識別子 | https://www.sciencedirect.com/science/article/am/pii/S1050464822004211 | |||||
関連名称 | Author's Accepted Manuscript Version/PDF (Available on: 2023-07-21) | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-25292125/ | |||||
関連名称 | KAKEN研究課題: 人工モノクローナル魚類抗体の迅速作出法の開発およびその応用 | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18H03958/ | |||||
関連名称 | KAKEN研究課題: 軟骨魚類の自然抗体を応用した魚類感染症の新規防除法の開発 | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000020314635/ | |||||
関連名称 | 研究代表者: 近藤 秀裕 (東京海洋大学) | |||||
情報源 | ||||||
関連識別子 | https://www.elsevier.com/ja-jp | |||||
関連名称 | Elsevier B.V. | |||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
科学研究費研究課題 | ||||||
主題 | 人工モノクローナル魚類抗体の迅速作出法の開発およびその応用 | |||||
科学研究費研究課題 | ||||||
主題 | Development and application of artificial monoclonal fish antibody | |||||
科学研究費研究課題 | ||||||
主題 | 軟骨魚類の自然抗体を応用した魚類感染症の新規防除法の開発 | |||||
科学研究費研究課題 | ||||||
主題 | Application of natural antibodies from cartilaginous fish | |||||
研究課題番号 | ||||||
内容記述 | 25292125 | |||||
研究課題番号 | ||||||
内容記述 | 18H03958 |