{"created":"2023-07-25T09:59:16.963607+00:00","id":2571,"links":{},"metadata":{"_buckets":{"deposit":"55e59c06-6e8a-472a-a8c4-a500e8b3249f"},"_deposit":{"created_by":15,"id":"2571","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2571"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002571","sets":["187:189:237","187:189:401","187:189:553","41:427","70:682","70:701"]},"author_link":["2000","2908","4071","2005","3213"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"947","bibliographicPageStart":"939","bibliographicVolumeNumber":"127","bibliographic_titles":[{"bibliographic_title":"Fish & Shellfish Immunology"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The structures of fish serum immunoglobulin differ among different fish species. In this study, we accidently isolated a rabbit immunoglobulin (Ig) light chain bound to serum immunoglobulin from different marine fish species using phage display. Fish Ig was separated using a protein A column.\nThe phage library was generated from variable regions of rabbit spleen B cells immunized with bluefin tuna Thunnus orientalis Ig. Fish Ig–specific phages were enriched using two rounds of bio-panning with yellowtail Seriola quinqueradiata serum Ig, followed by two rounds of bio-panning with red seabream Pagrus major serum Ig.\nThe enriched phages demonstrated an increase in binding specificity to the tuna, yellowtail, and red seabream Igs compared to the phages listed in the unpanned library.\nA recombinant protein of a single clonal phage, which encodes the rabbit Ig light chain, was produced, and the binding specificities to fish Igs were analyzed using enzyme-linked immunosorbent assay (ELISA) and western blotting.\nThe recombinant protein exhibited binding properties to fish Igs in the ELISA. However, the recombinant protein that bound to serum protein(s), but not IgM, was detected via western blotting.\nThe recombinant protein may provide a novel information on the common structural feature in the fish immunoglobulins."}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"公開日: 2023-07-21"}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"25292125"},{"subitem_description":"18H03958"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_3_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{}}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.fsi.2022.07.032","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Author's Accepted Manuscript Version/PDF (Available on: 2023-07-21) "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S1050464822004211","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 人工モノクローナル魚類抗体の迅速作出法の開発およびその応用"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-25292125/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 軟骨魚類の自然抗体を応用した魚類感染症の新規防除法の開発"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18H03958/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 近藤 秀裕 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000020314635/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Elsevier B.V."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.elsevier.com/ja-jp","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2022 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.fsi.2022.07.032"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1050-4648","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10838941","subitem_source_identifier_type":"NCID"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"人工モノクローナル魚類抗体の迅速作出法の開発およびその応用"},{"subitem_subject":"Development and application of artificial monoclonal fish antibody"},{"subitem_subject":"軟骨魚類の自然抗体を応用した魚類感染症の新規防除法の開発"},{"subitem_subject":"Application of natural antibodies from cartilaginous fish"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jirapongpairoj, Walissara"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nozaki, Reiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koiwai, Keiichiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hirono, Ikuo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kondo, Hidehiro"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Phage display","subitem_subject_scheme":"Other"},{"subitem_subject":"Library","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunoglobulin","subitem_subject_scheme":"Other"},{"subitem_subject":"Antibody","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Identification of a rabbit Ig light chain recombinant protein bound to serum immunoglobulins from different marine fish species","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of a rabbit Ig light chain recombinant protein bound to serum immunoglobulins from different marine fish species"}]},"item_type_id":"3","owner":"15","path":["237","401","427","553","682","701"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-21"},"publish_date":"2022-11-21","publish_status":"0","recid":"2571","relation_version_is_last":true,"title":["Identification of a rabbit Ig light chain recombinant protein bound to serum immunoglobulins from different marine fish species"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:28:38.813895+00:00"}