{"created":"2023-07-25T09:57:26.167886+00:00","id":353,"links":{},"metadata":{"_buckets":{"deposit":"c390eb96-657b-4413-86ab-7a897b39e433"},"_deposit":{"created_by":3,"id":"353","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"353"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00000353","sets":["187:188:224","89:128:137"]},"author_link":["1581","1020","1015","1018","1019","1017"],"item_6_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ナメクジウオmyc遺伝子の進化的解析"}]},"item_6_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-27","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"16","bibliographicPageStart":"11","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"東京海洋大学研究報告"}]}]},"item_6_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The proto-oncogene myc is one of the most important genes controlling cell proliferation. The vertebrate genome has four myc genes (c-, N-, L-, s-myc), whose evolutionary origin and relationship are unclear. Here, we isolated a myc gene from a protochordate, the amphioxus Branchiostoma belcheri, which is thought to be the nonvertebrate that is closest to the vertebrates. A 1480 bp cDNA sequence was determined and contains an ATGinitiated ORF consisting of 371 amino acids. The exon/intron structure was conserved. Southern blotting and degenerate PCR showed that the amphioxus genome contained only a single myc gene. A phylogenetic tree of Myc family genes based on the deduced amino acid sequences indicated that amphioxus Myc was located outside the vertebrate Myc family. These results suggest that myc gene duplication occurred after protochordate on phylogeny. ","subitem_description_type":"Abstract"},{"subitem_description":" がん原遺伝子myc は細胞増殖を制御する重要な遺伝子の一つである。脊椎動物では4種類のmycが存在しているが，その起源や類縁関係は明らかとなっていない。本研究で我々は，脊椎動物に最も近縁の無脊椎動物であるナメクジウオBranchiostoma belcheriからmyc遺伝子を単離した。全長1480塩基対のcDNA は371アミノ酸からなるORFを含んでいた。また，エキソン／イントロン構造は保存されていた。サザンブロット法およびdegenerate PCRの結果，ナメクジウオのゲノムは単一のmyc 遺伝子を持つことが明らかとなった。また，推定アミノ酸配列に基づいた系統解析の結果，ナメクジウオMyc は脊椎動物Mycファミリーの外側に位置していた。これらの結果から，myc ファミリーは原索動物以降に形成されたものと考えられた。","subitem_description_type":"Abstract"}]},"item_6_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"東京海洋大学海洋科学部海洋生物資源学科 ","subitem_description_type":"Other"},{"subitem_description":"東京海洋大学海洋科学部海洋生物資源学科 ","subitem_description_type":"Other"},{"subitem_description":"コネチカット大学 ","subitem_description_type":"Other"},{"subitem_description":"東京大学海洋研究所 ","subitem_description_type":"Other"},{"subitem_description":"日本魚類生物科学研究所 ","subitem_description_type":"Other"},{"subitem_description":"東京海洋大学海洋科学部海洋生物資源学科","subitem_description_type":"Other"}]},"item_6_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京海洋大学"}]},"item_6_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12066872","subitem_source_identifier_type":"NCID"}]},"item_6_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2189-0951","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 幹晴"},{"creatorName":"モリタ, ミキハル","creatorNameLang":"ja-Kana"},{"creatorName":"Morita, Mikiharu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"二見, 邦彦"},{"creatorName":"フタミ, クニヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Futami, Kunihiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"張, 寰"},{"creatorName":"Zhang, Huan","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"窪川, かおる"},{"creatorName":"クボカワ, カオル","creatorNameLang":"ja-Kana"},{"creatorName":"Kubokawa, Kaoru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小島, 吉雄"},{"creatorName":"オジマ, ヨシオ","creatorNameLang":"ja-Kana"},{"creatorName":"Ojima, Yoshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岡本, 信明"},{"creatorName":"オカモト, ノブアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Okamoto, Nobuaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-11-18"}],"displaytype":"detail","filename":"AA12066872-5-11.pdf","filesize":[{"value":"587.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA12066872-5-11.pdf","url":"https://oacis.repo.nii.ac.jp/record/353/files/AA12066872-5-11.pdf"},"version_id":"bfc4a799-703a-4267-862c-cd97f02e6999"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"protochordate","subitem_subject_scheme":"Other"},{"subitem_subject":"evolution","subitem_subject_scheme":"Other"},{"subitem_subject":"genome duplication","subitem_subject_scheme":"Other"},{"subitem_subject":"Myc family","subitem_subject_scheme":"Other"},{"subitem_subject":"vertebrate","subitem_subject_scheme":"Other"},{"subitem_subject":"原索動物","subitem_subject_scheme":"Other"},{"subitem_subject":"進化","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子重複","subitem_subject_scheme":"Other"},{"subitem_subject":"Myc ファミリー","subitem_subject_scheme":"Other"},{"subitem_subject":"脊椎動物","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Evolutionary analysis of amphioxus myc gene","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evolutionary analysis of amphioxus myc gene"}]},"item_type_id":"6","owner":"3","path":["137","224"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-05-08"},"publish_date":"2009-05-08","publish_status":"0","recid":"353","relation_version_is_last":true,"title":["Evolutionary analysis of amphioxus myc gene"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-25T10:54:41.145616+00:00"}