{"created":"2023-07-25T09:59:16.541243+00:00","id":2561,"links":{},"metadata":{"_buckets":{"deposit":"5c78e45b-1b10-4bf6-a466-713e6b933d6a"},"_deposit":{"created_by":15,"id":"2561","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2561"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002561","sets":["187:189:553","41:696"]},"author_link":["3500","4048","2908"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"130587","bibliographicVolumeNumber":"347","bibliographic_titles":[{"bibliographic_title":"Sensors and Actuators B : Chemical"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For analyzing the immune system of an organism, it is necessary to classify and separate distinct types of cells based on their characteristics.\nHemocytes, immune cells of shrimp, are still difficult to classify and separate with existing technologies due to the lack of obvious morphological characteristics or type-specific markers.\nWe adapted the deterministic lateral displacement (DLD) method as a tool to separate the hemocytes of shrimp because the previous work suggested there is a relevance between the size of hemocytes and their functions.\nThe image and flow cytometry (FCM) analyses confirmed that this microfluidics-based technology enabled the size-based separation of cells having a broad size distribution at the buffer condition suitable for the marine species.\nCells separated with this chip retained their morphological information, and qRT-PCR was successfully conducted to reveal the differences in expression levels of immune-related genes between cell populations of different sizes.\nThe present results prove that the DLD method can be an effective tool for the analysis of non-model organisms, in which the characteristics of cells are not well established and studied."}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"公開日: 2023-08-13"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.snb.2021.130587","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Author's Accepted Manuscript Version/PDF (Available on: 2023-08-13) "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S0925400521011552","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Elsevier B.V."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.elsevier.com/ja-jp","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2021 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.snb.2021.130587"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0925-4005","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1078104X","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Murakami, Tomoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koiwai, Keiichiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Suzuki, Hiroaki"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell separation","subitem_subject_scheme":"Other"},{"subitem_subject":"Microfluidics","subitem_subject_scheme":"Other"},{"subitem_subject":"Deterministic lateral displacement","subitem_subject_scheme":"Other"},{"subitem_subject":"Shrimp","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Applying deterministic lateral displacement cell separation on immune cells of Marine shrimp","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Applying deterministic lateral displacement cell separation on immune cells of Marine shrimp"}]},"item_type_id":"3","owner":"15","path":["553","696"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-09-26"},"publish_date":"2022-09-26","publish_status":"0","recid":"2561","relation_version_is_last":true,"title":["Applying deterministic lateral displacement cell separation on immune cells of Marine shrimp"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:29:13.643508+00:00"}