{"created":"2023-07-25T09:59:16.353287+00:00","id":2557,"links":{},"metadata":{"_buckets":{"deposit":"0f34200e-d160-468c-a631-c22cfd5f7562"},"_deposit":{"created_by":15,"id":"2557","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2557"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002557","sets":["134:580","187:190:694","187:191:236","187:191:245","41:584","70:628"]},"author_link":["4008","4042","4044","2178","1557","4043","4045","4046","4047"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageStart":"738490","bibliographicVolumeNumber":"560","bibliographic_titles":[{"bibliographic_title":"Aquaculture"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The albino mutation has been used as a visible genetic marker to evaluate offspring resulting from transplanting donor germ cells into recipients to produce donor-derived gametes in surrogates.\nUsing albino mutants as either recipients or donors makes it possible to easily identify the donor-derived offspring based on body color.\nThe chub mackerel (Scomber japonicus) is a promising recipient species for xenogeneic gamete production of commercially valuable scombrids such as bluefin tuna; however, no body-color mutants of this species have been reported.\nUsing the CRISPR/Cas9 system, we produced F1 albino chub mackerel harboring a biallelic null mutation in the solute carrier family 45a2 (slc45a2) gene.\nHowever, albino larvae ingested significantly less rotifers than wild-type larvae when reared with a normal feeding density of rotifers, resulting in zero albino larvae survival beyond 12 days post hatch.\nTo overcome this lethality, we developed a novel “spotlight method” of larviculture, in which positive phototaxis is used to attract both rotifers and larvae to create an ultra-high-density feeding area in a larval rearing tank.\nUnder these conditions, albino larvae ingested as much rotifers as wild-type larvae, thus improving albino larvae survival.\nFinally, we confirmed albino chub mackerel completely lack melanin pigmentation, indicating that we have developed a potentially useful recipient for germ cell transplantation in Scombridae species."}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"公開日: 2024-06-14"}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"20H00430"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.aquaculture.2022.738490","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Author's Accepted Manuscript Version/PDF (Available on: 2024-06-14) "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S0044848622006068","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 代理親魚を使っておいしい魚を育種する"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20H00430/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 吉崎 悟朗 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000070281003/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Elsevier B.V."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.elsevier.com/ja-jp","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2022 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.aquaculture.2022.738490"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0044-8486","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00543624","subitem_source_identifier_type":"NCID"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"代理親魚を使っておいしい魚を育種する"},{"subitem_subject":"Establishment of tasty fish strain using surrogate broodstock"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawamura, Wataru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasegawa, Niki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamauchi, Akihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yahagi, Hana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tani, Reoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Tetsuro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yazawa, Ryosuke"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yoshizaki, Goro"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CRISPR/Cas9","subitem_subject_scheme":"Other"},{"subitem_subject":"Phototactic behavior","subitem_subject_scheme":"Other"},{"subitem_subject":"Albino","subitem_subject_scheme":"Other"},{"subitem_subject":"Chub mackerel","subitem_subject_scheme":"Other"},{"subitem_subject":"Rotifer","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype"}]},"item_type_id":"3","owner":"15","path":["236","245","580","584","628","694"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-09-26"},"publish_date":"2022-09-26","publish_status":"0","recid":"2557","relation_version_is_last":true,"title":["Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:28:27.497597+00:00"}