@article{oai:oacis.repo.nii.ac.jp:00002557,
 author = {Kawamura, Wataru and Hasegawa, Niki and Yamauchi, Akihiro and Kimura, Takashi and Yahagi, Hana and Tani, Reoto and Morita, Tetsuro and Yazawa, Ryosuke and Yoshizaki, Goro},
 issue = {15},
 journal = {Aquaculture},
 month = {Nov},
 note = {The albino mutation has been used as a visible genetic marker to evaluate offspring resulting from transplanting donor germ cells into recipients to produce donor-derived gametes in surrogates.
Using albino mutants as either recipients or donors makes it possible to easily identify the donor-derived offspring based on body color.
The chub mackerel (Scomber japonicus) is a promising recipient species for xenogeneic gamete production of commercially valuable scombrids such as bluefin tuna; however, no body-color mutants of this species have been reported.
Using the CRISPR/Cas9 system, we produced F1 albino chub mackerel harboring a biallelic null mutation in the solute carrier family 45a2 (slc45a2) gene.
However, albino larvae ingested significantly less rotifers than wild-type larvae when reared with a normal feeding density of rotifers, resulting in zero albino larvae survival beyond 12 days post hatch.
To overcome this lethality, we developed a novel “spotlight method” of larviculture, in which positive phototaxis is used to attract both rotifers and larvae to create an ultra-high-density feeding area in a larval rearing tank.
Under these conditions, albino larvae ingested as much rotifers as wild-type larvae, thus improving albino larvae survival.
Finally, we confirmed albino chub mackerel completely lack melanin pigmentation, indicating that we have developed a potentially useful recipient for germ cell transplantation in Scombridae species., 公開日: 2024-06-14, 20H00430},
 title = {Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype},
 volume = {560},
 year = {2022}
}