@article{oai:oacis.repo.nii.ac.jp:00002556,
 author = {Imaizumi, Kentaro and Molex, Wanlapha and Jitnavee, Chakrit and Direkbusarakom, Sataporn and Kondo, Hidehiro and Hirono, Ikuo},
 issue = {30},
 journal = {Aquaculture},
 month = {May},
 note = {Although microorganisms in pond water and shrimp gut have been thought to be important factors in shrimp health, knowledge of the microbiota surrounding the shrimp is limited. In this study, we analyzed bacterial and eukaryotic microbiota in the pond water of whiteleg shrimp Litopenaeus vannamei, and bacterial compositions in the stomach and midgut of shrimp that are reared in the ponds.
Microorganisms were collected at three sites in each of two ponds using filter membranes for 7 consecutive days.
Amplicons of 16S rDNA and 18S rDNA were generated. Shrimp were also collected from the ponds on the same days and 16S rDNA was amplified from their stomachs and midguts and analyzed.
Results showed that the bacterial communities in the surface water were distributed uniformly and were relatively stable, and the differences between the ponds were small. 
However, a principal coordinate analysis indicated significant differences in the composition of bacteria in the water of each pond and the microbiota shifted continuously during the sampling period.
The eukaryotic plankton in the water changed dynamically and consecutively including the alternations of the dominant families in 1 to 2 days.
The bacterial compositions of the digestive tracts, especially the stomach, clearly differed between the two ponds.
Also, a bacterium, classified as Candidatus Bacilloplasma in class Mollicutes which was found in the stomachs might be a novel lineage of Mollicutes.
Analysis of the combination of bacterial and eukaryotic microbiota, and not only the intestine (midgut) but also the stomach microbiota would be useful to understand microbiome in shrimp aquaculture., 公開日: 2024-03-20},
 title = {Bacterial and eukaryotic communities in pond water of whiteleg shrimp Litopenaeus vannamei and the bacterial communities of their stomach and midgut},
 volume = {554},
 year = {2022}
}