{"created":"2023-07-25T09:59:15.467808+00:00","id":2538,"links":{},"metadata":{"_buckets":{"deposit":"e781d970-9c00-4b04-8959-3f2f5479ff50"},"_deposit":{"created_by":15,"id":"2538","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2538"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002538","sets":["187:188:224","187:189:679","187:190:550","41:428","70:678"]},"author_link":["4001","1581","7651","2070"],"control_number":"2538","item_3_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"104420","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"Developmental & Comparative Immunology"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Unlike most mammalian cell lines, fish cell lines are immortal and resistant to cellular senescence. Elevated expression of H-Ras contributes to the induction of senescence in a fish cell line, EPC, but is not sufficient to induce full senescence.\nHere, we focused on the absence of a p16INK4a/ARF locus in the fish genome, and investigated whether this might be a critical determinant of the resistance of EPC cells to full senescence. We found that transfected EPC cells constitutively overexpressing p16INK4a exhibited large size and flat morphology characteristic of prematurely senescent cells; the cells also showed p53-independent senescence-like growth arrest and senescence-associated β-galactosidase (SA-β-gal) activity. Furthermore, the mRNA levels of proinflammatory senescence-associated secretory phenotype (SASP) factors increased in EPC cells constitutively overexpressing p16INK4a.\nThese results suggest that the lack of p16INK4a in the fish genome may be a critical determinant of senescence resistance in fish cell lines.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"公開日: 2023-04-12","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"19K06234","subitem_description_type":"Other"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_3_relation_10":{"attribute_name":"item_3_relation_10","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"35417735","subitem_relation_type_select":"PMID"}}]},"item_3_relation_11":{"attribute_name":"item_3_relation_11","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.dci.2022.104420","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Author's Accepted Manuscript Version/PDF (Available on: 2023-04-12)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S0145305X22000829","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"KAKEN研究課題: 養殖環境のストレスはDNA脱メチル化を介して魚類細胞の老化を引き起こすか？"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K06234/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"研究代表者: 二見 邦彦 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000000513459/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Elsevier B.V."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.elsevier.com/ja-jp","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 202 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.dci.2022.104420","subitem_rights_language":"en"}]},"item_3_source_id_7":{"attribute_name":"item_3_source_id_7","attribute_value_mlt":[{"subitem_source_identifier":"0145-305X","subitem_source_identifier_type":"PISSN"}]},"item_3_source_id_9":{"attribute_name":"item_3_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"AA11525307","subitem_source_identifier_type":"NCID"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果","subitem_subject_scheme":"Other"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"養殖環境のストレスはDNA脱メチル化を介して魚類細胞の老化を引き起こすか？","subitem_subject_scheme":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Futami, Kunihiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Futami","familyNameLang":"en"}],"givenNames":[{"givenName":"Kunihiko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1581","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00513459","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00513459"}]},{"creatorNames":[{"creatorName":"Sato, Shunichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4001","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Maita, Masashi","creatorNameLang":"en"},{"creatorName":"舞田, 正志","creatorNameLang":"ja"},{"creatorName":"マイタ, マサシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Maita","familyNameLang":"en"},{"familyName":"舞田","familyNameLang":"ja"},{"familyName":"マイタ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Masashi","givenNameLang":"en"},{"givenName":"正志","givenNameLang":"ja"},{"givenName":"マサシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2070","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60238839","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60238839"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Katagiri, Takayuki","creatorNameLang":"en"},{"creatorName":"片桐, 孝之","creatorNameLang":"ja"},{"creatorName":"カタギリ, タカユキ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Katagiri","familyNameLang":"en"},{"familyName":"片桐","familyNameLang":"ja"},{"familyName":"カタギリ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Takayuki","givenNameLang":"en"},{"givenName":"孝之","givenNameLang":"ja"},{"givenName":"タカユキ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"7651","nameIdentifierScheme":"WEKO"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"5-aza-2′-deoxycytidine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EPC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SA-β-gal","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SASP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Senescence-like growth arrest","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Lack of a p16INK4a/ARF locus in fish genome may underlie senescence resistance in the fish cell line, EPC","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Lack of a p16INK4a/ARF locus in fish genome may underlie senescence resistance in the fish cell line, EPC","subitem_title_language":"en"}]},"item_type_id":"3","owner":"15","path":["428","678","224","679","550"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-09-12"},"publish_date":"2022-09-12","publish_status":"0","recid":"2538","relation_version_is_last":true,"title":["Lack of a p16INK4a/ARF locus in fish genome may underlie senescence resistance in the fish cell line, EPC"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2025-03-10T04:32:03.667648+00:00"}