@article{oai:oacis.repo.nii.ac.jp:00002508,
 author = {Hamasaki, Katsuyuki and Tsuboi, Takuya and Dan, Shigeki},
 journal = {水生動物, Aquatic Animals},
 month = {May},
 note = {The red swamp crayfish Procambarus clarkii is an invasive alien species worldwide. The sterile-male-release technique (SMRT), which uses sterile but sexually active males, may help control its population. Body sizes of males and females affect mating and spawning success through mate choice. 
However, mating behaviour and spawning between different-sized males and females of P. clarkii remain unclear. The present study elucidated the effects of body size on mating behaviour and spawning of P. clarkii to develop effective SMRT for this invasive species. We prepared nine test groups as a combination of males and females in three size classes, and the mating behaviour of the test pairs was videorecorded for 48 h; then, spawning and egg development rate were examined.
The body size difference negatively affected the copulation frequency and duration in the pairs, and the incidence of copulation was low in the pairs between the largest males and smallest females. When mating with smaller males, females delayed spawning and the egg development rate decreased, likely because the females may be waiting an opportunity to mate with larger males with higher reproductive potential. 
Our results might be consequences of mutual mate choice (i.e., preference for larger mates).
Our findings indicate that in SMRT, sterile medium- and large-sized males with high reproductive potential are recommended to satisfy the mutual mate choice in P. clarkii populations of various-sized females.},
 title = {Effects of body size on mating behaviour and spawning of the red swamp crayfish Procambarus clarkii},
 volume = {2022},
 year = {2022}
}