{"created":"2023-07-25T09:59:08.468416+00:00","id":2384,"links":{},"metadata":{"_buckets":{"deposit":"5633d4b3-c23f-4ffa-8743-3fb184e48ef5"},"_deposit":{"created_by":15,"id":"2384","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2384"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002384","sets":["187:189:555","41:618"]},"author_link":["1927","3699","3697","1991","3758"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"106","bibliographicPageStart":"99","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"海洋深層水研究"},{"bibliographic_title":"Deep Ocean Water Research","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"カルシウム／マグネシウム摂取比（以後，Ca/Mg比）の増大は虚血性心疾患のリスクを生じさせることは周知のとおりである。また動物実験によるCa/Mg比の増大と虚血性心疾患についての報告はあるが，Ca/Mg比に関する細胞レベルの研究報告はほとんど見当たらない。そこで継代数が異なる培養線維芽細胞を用いて，Caおよび Mg のさまざまな割合の影響を調査した。\nその結果，継代数が14回の細胞（老化したと考えられる細胞）では Ca/Mg比が高い条件で培養すると細胞活性が顕著に低下した。この現象は本研究で使用された範囲内でのCa濃度の増加にかかわりなくCa/Mg 比に依存することが示唆された。しかし，Ca/Mg比の増大に伴って細胞活性が低下する条件において，Ca/Mg比を変えない量の伊豆赤沢産の海洋深層水（以後，DSW）を添加して培養した結果，細胞活性の低下が阻止されることが示唆された。\n本研究の結果から，食の多様化に伴ってCa/Mg 比が増加傾向にある日本の食事に対してDSW の利用は健康維持上有用な一つのアプローチになると思われる。"},{"subitem_description":"It has been discussed that the increase in the intake ratio of calcium and magnesium (Ca/Mg ratio) increases the risk of the ischemic heart disease. Though there are some reports on Ca/Mg ratio concerning the ischemic heart disease in animal experiments, there are few reports of experiments on cultured cells. Therefore, we investigated the influence of various ratios of Ca/Mg on the cell viability of cultured fibroblasts at various passage numbers.\nAs a result, cell viability of cultured fibroblasts at passage number 14 or more significantly decreased with rises in Ca/Mg ratios. Furthermore, it was shown that this phenomenon was related to Ca/Mg ratios and not to rising Ca concentrations used in this report. Additionally, it was shown that the supplementation of DSW collected from Izu-Akazawa, Japan which did not change the Ca/Mg ratio, to cells in a state of decreased cell viability due to the increased Ca/Mg ratios showed an inhibition against decreasing their cell viability. \nConsidering these results, it is suggested that the application of DSW will be a promising approach on health maintenance because the Ca/Mg ratio has been an increasing trend along with the diversification of the Japanese diet."}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"海洋深層水利用学会"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11174/dowas.15.99","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11174/dowas.15.99","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"海洋深層水利用学会"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.dowas.net/","subitem_relation_type_select":"URI"}}]},"item_3_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"130007872198","subitem_relation_type_select":"NAID"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-8477","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11858274","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山田, 勝久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 正宏"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野村, 道康"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柴田, 雄次"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"今田, 千秋"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"カルシウム","subitem_subject_scheme":"Other"},{"subitem_subject":"マグネシウム","subitem_subject_scheme":"Other"},{"subitem_subject":"線維芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞活性","subitem_subject_scheme":"Other"},{"subitem_subject":"海洋深層水","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Magnesium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fibroblast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell-viability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Deep seawater","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"種々のカルシウム／マグネシウム比で培養したヒト線維芽細胞の活性と海洋深層水添加効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"種々のカルシウム／マグネシウム比で培養したヒト線維芽細胞の活性と海洋深層水添加効果"},{"subitem_title":"Cell viability of human broblasts cultured with various ratios of calcium and magnesium and eects of deep seawater supplementation","subitem_title_language":"en"}]},"item_type_id":"3","owner":"15","path":["555","618"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-07"},"publish_date":"2022-04-07","publish_status":"0","recid":"2384","relation_version_is_last":true,"title":["種々のカルシウム／マグネシウム比で培養したヒト線維芽細胞の活性と海洋深層水添加効果"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:32:08.698192+00:00"}