{"created":"2023-07-25T09:59:08.327618+00:00","id":2381,"links":{},"metadata":{"_buckets":{"deposit":"4247ee2e-36aa-496f-a8df-8ce9575dad37"},"_deposit":{"created_by":15,"id":"2381","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2381"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002381","sets":["187:189:555","41:618"]},"author_link":["3697","1991","3766","2513","3695"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"176","bibliographicPageStart":"167","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"海洋深層水研究"},{"bibliographic_title":"Deep Ocean Water Research","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"日本列島の岩手県から沖縄県および東シナ海の海底堆積物・生物・海浜砂試料から27℃で分離培養した放線菌合計611株のコレステロールエステラーゼ(CHE)生産菌を探索し, 300株の陽性株をヒット率(供試菌株数に対する陽性菌数の割合)は49, 1%であった。最高ヒット率海域は千葉県館山湾で74, 3%(35株中26株), 分離サンプルでは,海岸砂のヒット率が67, 8%(90株中61(株)と最高であった。これら陽性300株中CHE高活性3株(いずれも海底堆積物)が得られた。\n東京湾(2009年5月水深5mA株と命名), 東京湾(2009年11月水深20m,B株と命名)東シナ海(2009年10月水深300m, C株と命名)これら3株の16SrDNAの塩基配列解析から, A, B株はともにStreptomyces rubrolavendulae, またC株はS. glaucinigerと高い相同性を示した。"},{"subitem_description":"A total of 611 cholesterol esterase (CHE) producing actinomycete strains were isolated and characterized from marine bottom sediments, marine organisms and beach sands collected from bays coastal and offshore waters, and beaches facing the Pacific and the East China Sea around Japan. Three hundred positive strains exhibiting CHE activity were isolated. The percentage of positive strain (hit rate) was 49.1 %. Highest hit rate of 74.3 % (26 positive strains out of 35 strains) was observed in Tateyama Bay in Chiba Prefecture. Among the various samples, the highest hit rate of 678% (61 positive strains out of 90 strains) was observed in the samples from coastal sand. Among the 300 positive strains.\n3 strains which had high CHE activity were selected and used for further studies. Two strains named A and B were isolated from sediment samples from Tokyo Bay in May (5 m) and October 2009 (20 m), respectively, whereas one strain named C was isolated from the sediment sample obtained from the East China Sea (300 m). From the 16S rDNA analysis, strains A and B showed high homology with Streptomyces rubrolavendulae whereas strain C was close to S. glauciniger. Among the three, strain B showed stable highest CHE activity and was selected for the purification and characterization of the enzyme. The CHE was isolated from the culture supernatant of strain B by ammonium sulfate precipitation, anion exchange chromatography and gel filtration. The final preparation showed a single band on native electrophoresis. The molecular weight of the purified CHE was 62.2 kDa. The CHE exists as dimer forms composed of 22.7 kDa and 39,5 kDa in water solution. The CHE was stable up to 70°C and also showed pH stability from 4 to 10. The enzyme decomposed substrates such as linoleic acid cholesterol and oleic acid cholesterol. "}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"海洋深層水利用学会"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11174/dowas.14.167","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11174/dowas.14.167","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"海洋深層水利用学会"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dowas.net/index.html","subitem_relation_type_select":"URI"}}]},"item_3_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"130007863856","subitem_relation_type_select":"NAID"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-8477","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11858274","subitem_source_identifier_type":"NCID"}]},"item_3_text_61":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"カイヨウ カンキョウ カラ ノ コレステロール エステラーゼ セイサン ホウセンキン ノ ブンリ ト ショセイジョウ"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今田, 千秋"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"小野寺, かおる"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"寺原, 猛"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 武志"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"山田, 勝久"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"コレステロールエステラーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"海洋","subitem_subject_scheme":"Other"},{"subitem_subject":"放線菌","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptomyces","subitem_subject_scheme":"Other"},{"subitem_subject":"cholesterol esterase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"marine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"actinomycetes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptomyces","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"海洋環境からのコレステロールエステラーゼ生産放線菌の分離と諸性状","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"海洋環境からのコレステロールエステラーゼ生産放線菌の分離と諸性状"},{"subitem_title":"Isolation and Characterization of Cholesterol Esterase-Producing Actinomycetes from the Marine Environment","subitem_title_language":"en"}]},"item_type_id":"3","owner":"15","path":["555","618"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-05"},"publish_date":"2022-04-05","publish_status":"0","recid":"2381","relation_version_is_last":true,"title":["海洋環境からのコレステロールエステラーゼ生産放線菌の分離と諸性状"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:32:14.941319+00:00"}