{"created":"2023-07-25T09:59:08.095522+00:00","id":2376,"links":{},"metadata":{"_buckets":{"deposit":"6b84c22b-457e-4c43-acfb-cba7b2482054"},"_deposit":{"created_by":15,"id":"2376","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2376"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002376","sets":["187:189:555","41:618"]},"author_link":["3761","3697","1991","2513","3695"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"17","bibliographicPageStart":"11","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"海洋深層水研究"},{"bibliographic_title":"Deep Ocean Water Research","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"自然界でも微生物に関する研究が少ない海洋深層水(DSW)中には全く未知の、あるいは特異的な生理・生化学的諸性状を有する微生物の存在が期待されるが、微生物に関する基礎的知見は乏しいのが現状である。\nそこで、本研究ではDSW中に存在する微生物の中から、一般細菌および真菌を対象にして、変性剤濃度勾配ゲル電気泳動法(DGGE法)を用いて、微生物群集組成をそれぞれ調べた。その結果、年間を通じて様々な群衆の存在が示唆された。\nまた、溶存態有機物に付着している微生物(A)と遊離して存在している微生物(F)の群集組成は異なっていることがわかった。\nさらに、多次元尺度法(MDS法)で解析した結果、一般細菌および真菌ともに、群集組成は変遷することが示唆された。また、DGGEのバンドをシーケンス解析し、微生物を同定した結果、DSW中には属レベルで新規と思われる細菌や新規性が高いと思われる真菌の存在が示唆された。"},{"subitem_description":"Deep seawater (DSW), about which there are few microbial reports, is of interest as excellent sources of novel microorganisms or ones with unique natures; however, only a few studies have been undertaken to investigate the microbial community in DSW.  \nIn this study, we examined the community structures of bacteria and fungi in DSW samples collected from the pumping stations for DSW in Izu-Akazawa in Shizuoka Prefecture by denaturing gradient gel electrophoresis (DGGE) analyses. DGGE analyses revealed that several kinds of bacteria and fungi were present in DSW and he microbial community structures of attached microbes and free-living microbes differed.\nIn addition, multi-dimensional scaling (MDS) analysis of the obtained DGGE profiles indicated that each community structure of bacteria and fungi changed throughout the year with the exception of a few months.\nFurthermore, sequence analyses of the obtained DGGE profiles to identify microorganisms indicated that bacteria belonging to new genera and novel fungi were present in DSW samples collected from the pumping stations in Izu-Akazawa. "}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"海洋深層水利用学会"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11174/dowas.15.11","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.11174/dowas.15.11","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"海洋深層水利用学会"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.dowas.net/index.html","subitem_relation_type_select":"URI"}}]},"item_3_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"130007863854","subitem_relation_type_select":"NAID"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1345-8477","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11858274","subitem_source_identifier_type":"NCID"}]},"item_3_text_61":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"DGGEホウ ニ ヨル カイヨウ シンソウスイチュウ ノ ビセイブツ グンシュウ ソセイ カイセキ"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺原, 猛"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 貴大"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山田, 勝久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 武志"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"今田, 千秋"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"一般細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"海洋深層水","subitem_subject_scheme":"Other"},{"subitem_subject":"変性剤濃度勾配ゲル電気泳動法(DGGE法)","subitem_subject_scheme":"Other"},{"subitem_subject":"真菌","subitem_subject_scheme":"Other"},{"subitem_subject":"微生物群集組成","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacteria","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Deep seawater","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Denaturing gradient gel electrophoresis (DGGE) analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fungi","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microbial community structure","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"DGGE法による海洋深層水中の微生物群集組成解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DGGE法による海洋深層水中の微生物群集組成解析"},{"subitem_title":"Microbial Community Analysis of Deep Seawater by Denaturing Gradient Gel Electrophoresis","subitem_title_language":"en"}]},"item_type_id":"3","owner":"15","path":["555","618"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-05"},"publish_date":"2022-04-05","publish_status":"0","recid":"2376","relation_version_is_last":true,"title":["DGGE法による海洋深層水中の微生物群集組成解析"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:32:17.356707+00:00"}