{"created":"2023-07-25T09:59:06.960175+00:00","id":2355,"links":{},"metadata":{"_buckets":{"deposit":"7761c184-875d-43d3-88c3-3df0936b84ae"},"_deposit":{"created_by":15,"id":"2355","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2355"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002355","sets":["187:189:555","187:189:558","41:206"]},"author_link":["786","1991","269","2513","3716"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"402","bibliographicPageStart":"395","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"Fisheries Science"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Protoplast fusion between different species of Streptomyces was performed using a liquid regeneration method developed for a rapid and simple preparation of the fusants. Consequently, new clones, which could not be obtained using the conventional agar regeneration method, were obtained. In the crosses between S. griseus and S. durhamensis, and between S. californicus and S. catenulae, eight and two recombinants, respectively, were obtained using the liquid regeneration method. Conversely, in the case of crosses between S. ornatus and S. catenulae, and between S. ornatus and S. vendargensis, seven recombinants each were obtained using only the agar method. The physiological characteristics, such as the assimilation of carbohydrate and antibiotic resistance, of these fusants differed considerably from those of their parental strains. Using the proposed liquid regeneration method, a simpler and quicker procedure for protoplast fusion is described."}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本水産学会"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1046/j.1444-2906.2002.00438.x","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1046/j.1444-2906.2002.00438.x","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"日本水産学会"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://jsfs.jp/","subitem_relation_type_select":"URI"}}]},"item_3_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10008273357","subitem_relation_type_select":"NAID"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0919-9268","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10993718","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Imada, Chiaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Ikemoto, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Takeshi"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI"}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"佐藤, 奈保子 ","creatorNameLang":"ja"},{"creatorName":"サトウ, ナオコ","creatorNameLang":"ja"},{"creatorName":"Hamada, Naoko","creatorNameLang":"en"},{"creatorName":"濱田, 奈保子 ","creatorNameLang":"ja"},{"creatorName":"ハマダ, ナオコ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyNameLang":"ja"},{"familyNameLang":"ja"},{"familyNameLang":"en"},{"familyNameLang":"ja"},{"familyNameLang":"ja-Kana"}],"givenNames":[{"givenNameLang":"ja"},{"givenNameLang":"ja"},{"givenNameLang":"en"},{"givenNameLang":"ja"},{"givenNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Watanabe, Etsuo"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"fusion","subitem_subject_scheme":"Other"},{"subitem_subject":"protoplast","subitem_subject_scheme":"Other"},{"subitem_subject":"recombinant","subitem_subject_scheme":"Other"},{"subitem_subject":"regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptomyces","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method"}]},"item_type_id":"3","owner":"15","path":["206","555","558"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-29"},"publish_date":"2022-03-29","publish_status":"0","recid":"2355","relation_version_is_last":true,"title":["Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-04-02T08:13:07.227982+00:00"}