{"created":"2023-07-25T09:59:06.232983+00:00","id":2343,"links":{},"metadata":{"_buckets":{"deposit":"3e069648-fed6-4ffb-815f-8699284c2ae4"},"_deposit":{"created_by":15,"id":"2343","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2343"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002343","sets":["187:189:555","41:318"]},"author_link":["1991","2513","3695","3694"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"378","bibliographicPageStart":"371","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"日本水産学会誌"},{"bibliographic_title":"Nippon Suisan Gakkaishi","bibliographic_titleLang":"en"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"日本近海の海底堆積物および陸土壌から分離した 605 株の放線菌についてアルギン酸リアーゼ（A.L.）活性を調べた。その結果，18 株の有望株が得られ，これらの株について 16S rDNA の塩基配列から近縁種と性状の比較を行った。このうち大槌湾の水深 100 メートルから分離した Micromonospora sp. OB40608 株が最も高くしかも安定した活性を有していたため，A.L. を培養液上清から単離精製し，諸性状を明らかにした。本酵素の分子量はポリアクリルアミドゲル電気泳動（native-PAGE）で調べた結果，114 kDa であり，至適温度は 50°C で，至適 pH は 7.0 であった。"},{"subitem_description":"A total of 605 actinomycetes were isolated from marine and terrestrial sediments of various Japanese coastal areas using several isolation media. The largest number of alginate lyase (A.L.)-producing strains were isolated from marine sediment samples collected in Otsuchi Bay in Iwate Prefecture. The activities of 18 A.L.-producing strains were measured using liquid culture media and were identified by 16S rDNA sequencing analysis. Among the 18 strains, one species belonging to the genus of <i>Micromonospora</i> (named as strain OB40608, isolated from Otsuchi Bay at a depth of 100 m) showed high A.L. activity and has never been reported as an A.L. producer, therefore it was selected for further study. A.L. was purified from culture supernatant of strain OB40608 by ammonium sulfate precipitation, cation exchange chromatography (Cellufine C-500 m) and gel filtration chromatography (Sephacryl S-200 HR). The final preparation showed a single band on polyacryl amide gel electrophoresis. The molecular weight of purified A.L. was 114 kDa, and the optimum temperature and pH were 50℃ and pH 7.0, respectively."}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本水産学会"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2331/suisan.80.371","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2331/suisan.80.371","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"日本水産学会誌"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://jsfs.jp/","subitem_relation_type_select":"URI"}}]},"item_3_relation_28":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"130004451285","subitem_relation_type_select":"NAID"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-5392","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00193422","subitem_source_identifier_type":"NCID"}]},"item_3_text_61":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"カイヨウ カンキョウ カラ ノ アルギンサン リアーゼ セイサン ホウセンキン ノ ブンリ ト リアーゼ ノ セイセイ"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今田, 千秋"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"阪田, 圭"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"寺原, 猛"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小林, 武志"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Micromonospora","subitem_subject_scheme":"Other"},{"subitem_subject":"アルギン酸リアーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"海洋","subitem_subject_scheme":"Other"},{"subitem_subject":"放線菌","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"海洋環境からのアルギン酸リアーゼ生産放線菌の分離とリアーゼの精製","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"海洋環境からのアルギン酸リアーゼ生産放線菌の分離とリアーゼの精製"},{"subitem_title":"Isolaion of alginate lyase-producing actinomycetes from the marine environment and purification of lyase","subitem_title_language":"en"}]},"item_type_id":"3","owner":"15","path":["318","555"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-29"},"publish_date":"2022-03-29","publish_status":"0","recid":"2343","relation_version_is_last":true,"title":["海洋環境からのアルギン酸リアーゼ生産放線菌の分離とリアーゼの精製"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:32:40.349395+00:00"}