{"created":"2023-07-25T09:59:05.090410+00:00","id":2321,"links":{},"metadata":{"_buckets":{"deposit":"9d80f9f4-e4ba-4432-b600-0519a88cca22"},"_deposit":{"created_by":15,"id":"2321","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2321"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002321","sets":["187:190:445","41:584","70:559"]},"author_link":["3522","3608","3609"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-01-30","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"737496","bibliographicVolumeNumber":"547","bibliographic_titles":[{"bibliographic_title":"Aquaculture"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ergothioneine transporter (ETT) plays an important role in the incorporation of dietary ergothioneine (ET), a potent hydrophilic antioxidant produced intensively in certain edible mushroom species, in living organisms. We recently characterized ETT in rainbow trout (Oncorhynchus mykiss). However, the investigation of ETT in other commercially important salmonids is lacking. The present study aimed to provide molecular information on putative ETTs in coho salmon (Oncorhynchus kisutch), Atlantic salmon (Salmo salar), and chinook salmon (Oncorhynchus tshawytscha), particularly bioinformatic identification, gene expression in blood cells, muscle, intestine, and liver, which is responsible for deposition of ET in muscle, and development of universal primer set for quantitative real-time polymerase chain reaction (qPCR). In addition, ET concentration in these salmonids was investigated. The high sequence identity of putative ETT genes (E1s) to the characterized ETT orthologs and their placement under the ETT clade in the phylogenetic tree indicated that E1s are the putative ETTs of salmonids. The relationship between putative ETT gene expression ratio and the corresponding ET concentrations in the blood cells (whole blood for ET concentration), liver, intestine, and muscle suggested that the putative ETTs should act as ETTs. Species-specific primer sets and a universal primer set applicable for qPCR of these salmonid species were successfully developed and used for quantitative gene expression analysis. The present study successfully provides genomic information on the putative ETTs, and a universal primer set that is advantageous for detecting the expression of putative ETT genes in edible fish species. The expression of putative ETT genes highlights the potential of ET supplementation in future aquaculture of salmonids as a strategy to prevent quality deterioration due to lipid oxidation and discoloration of their flesh during storage."}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"公開予定日: 2023-09-23"}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"19K06235"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.aquaculture.2021.737496","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Author's Accepted Manuscript Version/PDF (Available on: 2023-09-23)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S0044848621011595","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 魚類筋肉pH調整に及ぼす食餌性エルゴチオネインの影響"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K06235/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 大島 敏明 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000070134856/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Elsevier B.V."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.elsevier.com/ja-jp","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2021 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.aquaculture.2021.737496"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0044-8486","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"魚類筋肉pH調整に及ぼす食餌性エルゴチオネインの影響"},{"subitem_subject":"Effects of dietary ergothionein consumption on physiological pH of fish muscle"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitsanayanyong, Lalitphan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohshima, Toshiaki"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ergothioneine","subitem_subject_scheme":"Other"},{"subitem_subject":"Putative ergothioneine transporter","subitem_subject_scheme":"Other"},{"subitem_subject":"Real-time qPCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Salmonids","subitem_subject_scheme":"Other"},{"subitem_subject":"Universal primers","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Putative ergothioneine transporter of salmonids as a functional tool for ergothioneine supplementation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Putative ergothioneine transporter of salmonids as a functional tool for ergothioneine supplementation"}]},"item_type_id":"3","owner":"15","path":["445","559","584"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-09"},"publish_date":"2022-03-09","publish_status":"0","recid":"2321","relation_version_is_last":true,"title":["Putative ergothioneine transporter of salmonids as a functional tool for ergothioneine supplementation"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:19:03.322865+00:00"}