{"created":"2023-07-25T09:59:00.624818+00:00","id":2233,"links":{},"metadata":{"_buckets":{"deposit":"5c9c197c-e9d1-47a8-aeae-cb26d1f134df"},"_deposit":{"created_by":15,"id":"2233","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2233"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002233","sets":["187:189:561","187:190:445","41:560","70:559"]},"author_link":["2116","3522","3523","2556","3524","3244"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-02","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"256","bibliographic_titles":[{"bibliographic_title":"Comparative Biochemistry and Physiology Part B : Biochemistry and Molecular Biology"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(2S)-3-(2-sulfanylidene-1,3-dihydroimidazol-4-yl)-2-(trimethylazaniumyl)propanoate (ergothioneine, ET) is hy-drophilic antioxidant produced only by certain low-level organisms, but has been reported in the organs of some animals upon dietary intake, facilitated by the ergothioneine transporter (ETT). In this study, it was hypothesised that ETT is involved in the uptake of ET from diet in salmonids. The mechanism of ET uptake was evaluated through knockdown of candidate gene encoding ETT in a cell line (RTG-2) from rainbow trout (Oncorhynchus mykiss). ETT gene expression in blood cells and certain tissues from rainbow trout was also investigated as ET from the diet is expected to be carried over to the muscle. Knockdown of an ETT candidate (E1) in RTG-2 cells resulted in a remarkable decrease in E1 mRNA expression; uptake of ET was lower in the knocked group than in the control group suggesting that E1 functions as an ETT for rainbow trout. Furthermore, the expression of ETT gene in different tissues including the muscle points to ET uptake from the diet to the muscle of salmonids. In conclusion, this study partly reveals the possible pathway underlying ET uptake from the diet and its deposition in the muscle of edible fish species. "}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"公開予定日：2022-06-05"}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"19K06235"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Science"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.cbpb.2021.110631","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Author's Accepted Manuscript Version/PDF (Available on: 2022-06-05)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/am/pii/S1096495921000701","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 魚類筋肉pH調整に及ぼす食餌性エルゴチオネインの影響"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19K06235/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 大島敏明 (東京海洋大学) "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000070134856/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Elsevier B. V."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.elsevier.com/ja-jp","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2020 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.cbpb.2021.110631"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1096-4959","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"魚類筋肉pH調整に及ぼす食餌性エルゴチオネインの影響"},{"subitem_subject":"Effects of dietary ergothionein consumption on physiological pH of fish muscle"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitsanayanyong, Lalitphan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Pahila, Jade"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koyama, Tomoyuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kiron, Viswanath"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohshima, Toshiaki"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ergothioneine","subitem_subject_scheme":"Other"},{"subitem_subject":"Ergothioneine transporter","subitem_subject_scheme":"Other"},{"subitem_subject":"Mushroom extract","subitem_subject_scheme":"Other"},{"subitem_subject":"Rainbow trout knockdown","subitem_subject_scheme":"Other"},{"subitem_subject":"RTG-2","subitem_subject_scheme":"Other"},{"subitem_subject":"Salmonids","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Functional identification of ergothioneine transporter in rainbow trout (Oncorhynchus mykiss)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Functional identification of ergothioneine transporter in rainbow trout (Oncorhynchus mykiss)"}]},"item_type_id":"3","owner":"15","path":["445","559","560","561"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-27"},"publish_date":"2021-09-27","publish_status":"0","recid":"2233","relation_version_is_last":true,"title":["Functional identification of ergothioneine transporter in rainbow trout (Oncorhynchus mykiss)"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:19:02.490229+00:00"}