@article{oai:oacis.repo.nii.ac.jp:00002225,
 author = {Futami, Kunihiko and Furukawa, Oto and Maita, Masashi and Katagiri, Takayuki},
 issue = {4},
 journal = {Fish Pathology},
 note = {Recently, our lab reported that CUBIC, a tissue-clearing technique, could help reveal the initial route of infection, its spread, and the localization of pathogens in infected goldfish Carassius auratus. ​However, this technique, in its original form, failed to clear melanin pigmented parts of the fish. ​We show here that bleaching with H2O2 clears melanin pigments of Black Moor goldfish without causing severe histological damage. ​Furthermore, nuclear staining with PI helps visualize the internal structures of cleared fish in a dark field. ​Since bleaching does not significantly quench the fluorescence of GFP, it may be applied to the in vivo imaging of GFP.},
 pages = {101--103},
 title = {Application of Hydrogen Peroxide-Melanin Bleaching and Fluorescent Nuclear Staining for Whole-Body Clearing and Imaging in Fish},
 volume = {54}
}