{"created":"2023-07-25T09:58:59.455736+00:00","id":2208,"links":{},"metadata":{"_buckets":{"deposit":"3b214889-c172-4865-af97-1772e87fe8be"},"_deposit":{"created_by":15,"id":"2208","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2208"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00002208","sets":["187:189:208","187:189:531","187:191:207","41:530","70:532"]},"author_link":["3445","3446","2577","1828","2994","768","3447"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-04-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Open Biology"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The long-standing paradigm establishing that global production of Omega-3 (n–3) long-chain polyunsaturated fatty acids (LC-PUFA) derived almost exclusively from marine single-cell organisms, was recently challenged by the discovery that multiple invertebrates possess methyl-end (or ωx) desaturases, critical enzymes enabling the biosynthesis of n–3 LC-PUFA. However, the question of whether animals with ωx desaturases have complete n–3 LC-PUFA biosynthetic pathways and hence can contribute to the production of these compounds in marine ecosystems remained unanswered. In the present study, we investigated the complete enzymatic complement involved in the n–3 LC-PUFA biosynthesis in Tigriopus californicus, an intertidal harpacticoid copepod. A total of two ωx desaturases, five front-end desaturases and six fatty acyl elongases were successfully isolated and functionally characterized. The T. californicus ωx desaturases enable the de novo biosynthesis of C18 PUFA such as linoleic and α-linolenic acids, as well as several n–3 LC-PUFA from n–6 substrates. Functions demonstrated in front-end desaturases and fatty acyl elongases unveiled various routes through which T. californicus can biosynthesize the physiologically important arachidonic and eicosapentaenoic acids. Moreover, T. californicus possess a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid via the ‘Δ4 pathway’. In conclusion, harpacticoid copepods such as T. californicus have complete n–3 LC-PUFA biosynthetic pathways and such capacity illustrates major roles of these invertebrates in the provision of essential fatty acids to upper trophic levels."}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"19K15908"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Royal Society"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1098/rsob.200402","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1098/rsob.200402","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: カイアシ類は多価不飽和脂肪酸の一次生産者として機能するか？"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?kw=19K15908","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 壁谷尚樹 (東京海洋大学) "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000090758731/","subitem_relation_type_select":"URI"}}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果 "}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"カイアシ類は多価不飽和脂肪酸の一次生産者として機能するか？"},{"subitem_subject":"The role of copepods as primary producers of polyunsaturated fatty acids"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kabeya, Naoki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Ogino, Masanari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ushio, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Haga, Yutaka"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Satoh, Shuichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Juan C. Navarro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Óscar Monroig"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"biosynthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"polyunsaturated fatty acids","subitem_subject_scheme":"Other"},{"subitem_subject":"fatty acyl elongases","subitem_subject_scheme":"Other"},{"subitem_subject":"front-end desaturases","subitem_subject_scheme":"Other"},{"subitem_subject":"methyl- end desaturases","subitem_subject_scheme":"Other"},{"subitem_subject":"harpacticoid copepods","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus"}]},"item_type_id":"3","owner":"15","path":["207","208","530","531","532"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-21"},"publish_date":"2021-09-21","publish_status":"0","recid":"2208","relation_version_is_last":true,"title":["A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n–3) exists in the marine Harpacticoida copepod Tigriopus californicus"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:34:14.636436+00:00"}