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CD4-1 and CD4-2 single positive are two major CD4 lymphocyte subpopulations in ginbuna crucian carp Carassius auratus langsdorfii
https://oacis.repo.nii.ac.jp/records/2000242
https://oacis.repo.nii.ac.jp/records/2000242570f6397-92a2-43d6-b2e4-ab57fc892488
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2024-09-02 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | CD4-1 and CD4-2 single positive are two major CD4 lymphocyte subpopulations in ginbuna crucian carp Carassius auratus langsdorfii | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | CD4-2, Lymphocytes, Ginbuna crucian carp, Monoclonal antibody | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| アクセス権 | ||||||||||||||||||||
| アクセス権 | metadata only access | |||||||||||||||||||
| 著者 |
Lau, Lik-ming
× Lau, Lik-ming
× Kuga, Misato
× 佐野, 元彦
× Kato, Goshi
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| 書誌情報 |
en : Fish & Shellfish Immunology 巻 138, p. 108785, 発行日 2023-07 |
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| 抄録 | ||||||||||||||||||||
| 内容記述 | In this study, we established a murine cell line that expresses ginbuna crucian carp (ginbuna) CD4-2 and used it to develop an anti-CD4-2 monoclonal antibody (mAb). An established mAb, named D5, showed good reactivities to BALB/c 3T3 cells expressing CD4-2 and a lymphocyte population in the ginbuna leukocytes. Gene expression analysis showed that D5+ cells express CD4-2 and TCRβ genes but not CD4-1 and IgM genes, meanwhile May Grunwald-Giemsa staining of sorted D5+ cells had the typical morphology of lymphocytes. Two-color immunofluorescence analysis with anti-CD4-1 mAb (6D1) and anti-CD4-2 mAb (D5) by flow cytometry revealed that the percentages of CD4-1 single positive (SP) and CD4-2 SP lymphocytes were comparatively higher than CD4-1/CD4-2 double positive (CD4 DP) lymphocytes in all tissues examined in ginbuna. The highest percentage of CD4-2 SP cells (?40%) was found in the thymus, while the head-kidney exhibited the highest percentages of CD4-1 SP (?30%) and CD4 DP (?5%) cells. These findings indicated that ginbuna CD4+ lymphocyte population consists of two major subpopulations (CD4-1 SP and CD4-2 SP) and a minor subset (CD4 DP). | |||||||||||||||||||
| 内容記述 | ||||||||||||||||||||
| 内容記述 | 公開日: 2023-03-13 | |||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||||
| 収録物識別子 | 1050-4648 | |||||||||||||||||||
| item_3_source_id_9 | ||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA10838941 | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.fsi.2023.108785 | |||||||||||||||||||
| 情報源 | ||||||||||||||||||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S1050464823002711 | |||||||||||||||||||
| 関連名称 | Author's Accepted Manuscript Version/PDF (Available on: 2023-03-13) | |||||||||||||||||||
| 情報源 | ||||||||||||||||||||
| 関連識別子 | https://www.elsevier.com/ja-jp | |||||||||||||||||||
| 関連名称 | Elsevier B.V. | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | Elsevier B.V. | |||||||||||||||||||
| 科学研究費研究課題 | ||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||
| 主題URI | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-22KJ1390/ | |||||||||||||||||||
| 主題 | 魚類独自のCD4-2陽性Ｔ細胞サブセットに関する研究 | |||||||||||||||||||
| 研究課題番号 | ||||||||||||||||||||
| 内容記述 | 20J22961 | |||||||||||||||||||
