{"created":"2023-07-25T09:58:45.389372+00:00","id":1881,"links":{},"metadata":{"_buckets":{"deposit":"23e184fe-2c4f-4e28-92bd-b5ca6880ed85"},"_deposit":{"created_by":15,"id":"1881","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"1881"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00001881","sets":["187:189:208","187:189:237","187:191:207","41:443","70:202"]},"author_link":["3122","2577","768","2000","3123"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-10-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"722","bibliographicPageStart":"714","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"AACL Bioflux"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Anxiety regarding the fate of ingested transgenic DNA in farmed fish fed genetically modified (GM) soybean meal (SBM) has been raised with regard to human consumption. The objective of this study was to detect possibility of gene transfer of transgenic and endogenous DNA fragments in Nile tilapia (Oreochromis niloticus) blood and organs after consumption of a GM SBM diet. Nile tilapias with an average weight of 75.0 g were fed diets containing 48% GM or non-GM SBM for 21 days. During this period, a GM SBM diet was fed to fish for 12 days, and then switched to feed with non-GM SBM for 9 days for determining the residual span of the transferred cauliflower mosaic virus (CaMV) 35S promoter fragment. Blood, spleen, liver, intestine, kidney, and muscle tissues were taken (n = 10) every three days during the feeding period. Total DNA was extracted from the samples and analyzed by polymerase chain reaction (PCR) for determining the presence of a 108-bp fragment of the CaMV 35S promoter and a 144-bp fragment of the soybean chloroplast-specific DNA. Low-copy chloroplast-specific DNA fragment was detected in all organ and tissue samples and the majority of intestinal samples of fish fed GM SBM diet. Similarly, a low number and faint signals of the CaMV 35S promoter fragments were detected in all organ samples except muscle of fish fed the GM SBM diet, while none were detected 3 days after changing to a non-GM SBM diet. A very low frequency of transmittance to muscle and organs of fish was confirmed. It is recognized that the low copy number of transgenic DNA in the GM SBM diet is a challenge to their detection in tissues. These results suggested that transgenic DNA would be processed in the gastrointestinal tract in a similar manner with conventional plant DNA."}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"26252034"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Bioflux Society"}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.bioflux.com.ro/home/volume-8-5-2015/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Bioflux Society"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.bioflux.com.ro/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 魚類の育成環境適応性と環境負荷低減を考慮した養魚飼料の基礎的研究"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-26252034/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 佐藤秀一 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000080154053/","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C)2015 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited."}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18449166","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"魚類の育成環境適応性と環境負荷低減を考慮した養魚飼料の基礎的研究"},{"subitem_subject":"Studies on the development of aquafeed with consideraton of rearing condition and low load to environment"}]},"item_3_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Indra Suharman"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Shuichi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Haga, Yutaka"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hirono, Ikuo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Zainal A. Muchlisin"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-03"}],"displaytype":"detail","filename":"kaken26252034-AACLB8_5_714.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"kaken26252034-AACLB8_5_714","url":"https://oacis.repo.nii.ac.jp/record/1881/files/kaken26252034-AACLB8_5_714.pdf"},"version_id":"aea1eeb5-df8c-4d80-91c1-28252eb62567"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"diet","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA transfer","subitem_subject_scheme":"Other"},{"subitem_subject":"genetically modified soybean","subitem_subject_scheme":"Other"},{"subitem_subject":"Nile tilapia","subitem_subject_scheme":"Other"},{"subitem_subject":"transgenic DNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Detection of transgenic and endogenous plant DNA in blood and organs of Nile tilapia, Oreochromis niloticus fed a diet formulated with genetically modified soybean meal","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of transgenic and endogenous plant DNA in blood and organs of Nile tilapia, Oreochromis niloticus fed a diet formulated with genetically modified soybean meal"}]},"item_type_id":"3","owner":"15","path":["202","207","208","237","443"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-09"},"publish_date":"2020-03-09","publish_status":"0","recid":"1881","relation_version_is_last":true,"title":["Detection of transgenic and endogenous plant DNA in blood and organs of Nile tilapia, Oreochromis niloticus fed a diet formulated with genetically modified soybean meal"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:17:42.555541+00:00"}