{"created":"2023-07-25T09:58:39.122000+00:00","id":1782,"links":{},"metadata":{"_buckets":{"deposit":"9d9fe5c1-0c67-4026-bfc8-3ed385edc0e9"},"_deposit":{"created_by":15,"id":"1782","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"1782"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00001782","sets":["187:189:237","187:189:401","41:276","70:399"]},"author_link":["2953","2000","2563","2908","2005"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-04-04","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"C-type lectins (CTLs) are calcium-dependent carbohydrate-binding proteins known to assist the innate immune system as pattern recognition receptors (PRRs). The binding specificity of CTLs lies in the motif of their carbohydrate recognition domain (CRD), the tripeptide motifs EPN and QPD bind to mannose and galactose, respectively. However, variants of these motifs were discovered including a QAP sequence reported in shrimp believed to have the same carbohydrate specificity as QPD. Here, we characterized a novel C-type lectin (MjGCTL) possessing a CRD with a QAP motif. The recombinant MjGCTL has a calcium-dependent agglutinating capability against both Gram-negative and Gram-positive bacteria, and its sugar specificity did not involve either mannose or galactose. In an encapsulation assay, agarose beads coated with rMjGCTL were immediately encapsulated from 0 h followed by melanization at 4 h post-incubation with hemocytes. These results confirm that MjGCTL functions as a classical CTL. The structure of QAP motif and carbohydrate-specificity of rMjGCTL was found to be different to both EPN and QPD, suggesting that QAP is a new motif. Furthermore, MjGCTL acts as a PRR binding to hemocytes to activate their adherent state and initiate encapsulation."}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"15H02462"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature Publishing AG"}]},"item_3_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{}}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep45818","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep45818","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: 病原微生物の侵入・攻撃に対するクルマエビ類の免疫応答に関する研究"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-15H02462/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 廣野 育生 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000000270926/","subitem_relation_type_select":"URI"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"病原微生物の侵入・攻撃に対するクルマエビ類の免疫応答に関する研究"},{"subitem_subject":"Immune response of kuruma shrimp against microbial infections"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Alenton, Rod Russel Reyes"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koiwai, Keiichiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Miyaguchi, Kohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Hidehiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hirono, Ikuo"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Glycobiology","subitem_subject_scheme":"Other"},{"subitem_subject":"Pattern recognition receptors","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif"}]},"item_type_id":"3","owner":"15","path":["237","276","399","401"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-06-24"},"publish_date":"2019-06-24","publish_status":"0","recid":"1782","relation_version_is_last":true,"title":["Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:43:41.990182+00:00"}