{"created":"2023-07-25T09:58:31.116591+00:00","id":1637,"links":{},"metadata":{"_buckets":{"deposit":"5026d572-9f71-4200-98a7-a785883fff1a"},"_deposit":{"created_by":15,"id":"1637","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"1637"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00001637","sets":["187:191:245","41:276","70:270"]},"author_link":["1937","2253","2714","1557"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-11-02","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Long-term preservation of fish fertility is essential for the conservation of endangered fishes. However, cryopreservation techniques for fish oocytes and embryos have not yet been developed. In the present study, functional eggs and sperm were derived from whole rainbow trout that had been frozen in a freezer and stored without the aid of exogenous cryoprotectants. Type A spermatogonia retrieved from frozen-thawed whole trout remained viable after freezing duration up to 1,113 days. Long-term-frozen trout spermatogonia that were intraperitoneally transplanted into triploid salmon hatchlings migrated toward the recipient gonads, where they were incorporated, and proliferated rapidly. Although all triploid recipients that did not undergo transplantation were functionally sterile, 2 of 12 female recipients and 4 of 13 male recipients reached sexual maturity. Eggs and sperm obtained from the salmon recipients were capable of producing donor-derived trout offspring. This methodology is thus a convenient emergency tool for the preservation of endangered fishes."}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"25114005"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature Publishing AG"}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/srep16045","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF (OpenAccess)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep16045","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: サケ科魚類の進化に伴うGSC制御機構の変化"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-25114005/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 吉崎 悟朗 (東京海洋大学)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000070281003/","subitem_relation_type_select":"URI"}}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果"}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"サケ科魚類の進化に伴うGSC制御機構の変化"},{"subitem_subject":"Evolution of GSC regulation in salmonids Planned"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lee, Seungki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shinsuke Seki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katayama, Naoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshizaki, Goro"}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Adult stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Self-renewal","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem-cell biotechnology","subitem_subject_scheme":"Other"},{"subitem_subject":"Transdifferentiation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Production of viable trout offspring derived from frozen whole fish","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of viable trout offspring derived from frozen whole fish"}]},"item_type_id":"3","owner":"15","path":["245","270","276"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-07"},"publish_date":"2019-01-07","publish_status":"0","recid":"1637","relation_version_is_last":true,"title":["Production of viable trout offspring derived from frozen whole fish"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:44:52.707182+00:00"}