{"created":"2023-07-25T09:58:30.811779+00:00","id":1630,"links":{},"metadata":{"_buckets":{"deposit":"6f8faedc-9183-48c0-9d46-486d81489414"},"_deposit":{"created_by":15,"id":"1630","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"1630"},"status":"published"},"_oai":{"id":"oai:oacis.repo.nii.ac.jp:00001630","sets":["187:191:245","41:271","70:270"]},"author_link":["1937","1557","2696"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"290","bibliographicPageStart":"286","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"Cryobiology"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years possessed the ability to differentiate into functional eggs or sperm in the gonads of triploid recipient masu salmon (O. masou). After cryopreservation for 5 years in liquid nitrogen, intraperitoneally transplanted spermatogonia migrated toward, and incorporated into, the gonads of xenogeneic recipients. The transplanted spermatogonia resumed spermatogenesis and oogenesis in male and female recipients, respectively, and differentiated into sperm or eggs within the gonads of male and female recipients at 2 years posttransplantation. The differentiated sperm and eggs generated normal rainbow trout representative of donor phenotypes. Thus, cryopreservation of spermatogonia is a powerful and reliable method for long-term preservation of fish genetic resources."}]},"item_3_description_63":{"attribute_name":"研究課題番号","attribute_value_mlt":[{"subitem_description":"25114005"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_3_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{}}]},"item_3_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.cryobiol.2016.08.001","subitem_relation_type_select":"DOI"}}]},"item_3_relation_13":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Publisher's Version/PDF "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.cryobiol.2016.08.001","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"KAKEN研究課題: サケ科魚類の進化に伴うGSC制御機構の変化"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-25114005/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"研究代表者: 吉崎 悟朗 (東京海洋大学) "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://nrid.nii.ac.jp/ja/nrid/1000070281003/","subitem_relation_type_select":"URI"}}]},"item_3_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2016 Elsevier Inc.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.cryobiol.2016.08.001"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00112240","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00620601","subitem_source_identifier_type":"NCID"}]},"item_3_subject_62":{"attribute_name":"件名","attribute_value_mlt":[{"subitem_subject":"科学研究費研究成果 "}]},"item_3_subject_64":{"attribute_name":"科学研究費研究課題","attribute_value_mlt":[{"subitem_subject":"サケ科魚類の進化に伴うGSC制御機構の変化"},{"subitem_subject":"Evolution of GSC regulation in salmonids Planned"}]},"item_3_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lee, Seungki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshiko Iwasaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshizaki, Goro"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-12-12"}],"displaytype":"detail","filename":"25114005_cryobiology_73-2-286.pdf","filesize":[{"value":"644.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"25114005_cryobiology_73-2-286","url":"https://oacis.repo.nii.ac.jp/record/1630/files/25114005_cryobiology_73-2-286.pdf"},"version_id":"bba8caf8-eba7-401a-b376-dda804602a2c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Rainbow trout","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Masu salmon","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Testis cryopreservation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Slow freezing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Spermatogonial stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interspecies transplantation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article"}]},"item_title":"Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids"}]},"item_type_id":"3","owner":"15","path":["245","270","271"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-07"},"publish_date":"2019-01-07","publish_status":"0","recid":"1630","relation_version_is_last":true,"title":["Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-25T10:44:58.660348+00:00"}