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Development of a polymerase chain reaction (PCR)-based genetic sex identification method in the chub mackerel Scomber japonicus and blue mackerel S. australasicus
https://oacis.repo.nii.ac.jp/records/2577
https://oacis.repo.nii.ac.jp/records/257780337936-f32b-485f-9c7d-3469e47d1c1c
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-11-21 | |||||
タイトル | ||||||
タイトル | Development of a polymerase chain reaction (PCR)-based genetic sex identification method in the chub mackerel Scomber japonicus and blue mackerel S. australasicus | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | Chub mackerel Scomber japonicus, Blue mackerel S. australasicus, Molecular sexing, Broodstock management, Pool-seq | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Tani, Reoto
× Tani, Reoto× Kawamura, Wataru× Morita, Tetsuro× Klopp, Christophe× Milhes, Marine× Guiguen, Yann× Yoshizaki, Goro× Yazawa, Ryosuke |
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書誌情報 |
Fisheries Science 巻 87, p. 785-793, 発行日 2021-08-30 |
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抄録 | ||||||
内容記述 | Chub mackerel Scomber japonicus and blue mackerel S. australasicus have become important aquaculture target species in Japan. For an efficient and stable aquaculture production of mackerels, it is desirable to work with a female-biased broodstock and thus to be able to manage precisely their sex ratio. As these two mackerel species do not have any external sexual dimorphism, direct biopsy of the gonads or measurement of sex hormone plasma levels is used in research to determine their phenotypic sex. However, these methods are associated with handling stress that results in gonadal atresia. Besides, it is difficult to identify sex using these methods in immature individuals. Therefore, genetic sexing would be desirable in these species to overcome these problems. In this study, we developed molecular sex identification tools for both chub and blue mackerels using sex-specific single-nucleotide variations (SNVs) identified by pool-sequencing (pool-seq) analyses. Genomic DNA was isolated from 30 males and females, in each species, and sequenced as pooled samples. Results of these pool-seq analyses identified a clear XX/XY male heterogametic sex-determination system in blue mackerel and a ZZ/ZW female heterogametic system in chub mackerel. Using this pool-seq information, we designed primer sets for genetic sex identification in both species. Results demonstrated that these primer sets are robust and accurate in assessing the genotypic sex of both blue and chub mackerels (accuracy > 99.5% in the chub, and > 94.5% in the blue mackerel) in various geographical samples of different ages. This method can now be applied for the efficient management of sex ratios in both mackerel aquaculture populations and wild-caught animals for fisheries management. |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0919-9268 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10993718 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s12562-021-01548-z | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1007/s12562-021-01548-z | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | https://www.kaiyodai.ac.jp/Japanese/IRBAS/index.html | |||||
関連名称 | 水圏生殖工学研究所 | |||||
情報源 | ||||||
関連識別子 | https://jsfs.jp/ | |||||
関連名称 | Japanese Society of Fisheries Science | |||||
情報源 | ||||||
関連識別子 | https://www.nature.com/ | |||||
関連名称 | Nature | |||||
出版者 | ||||||
出版者 | Springer Nature | |||||
出版者 | ||||||
出版者 | 日本水産学会 |