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Applying deterministic lateral displacement cell separation on immune cells of Marine shrimp
https://oacis.repo.nii.ac.jp/records/2561
https://oacis.repo.nii.ac.jp/records/2561ff40ff8f-eb9f-4582-906f-2cd92f8ad27c
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-09-26 | |||||
タイトル | ||||||
タイトル | Applying deterministic lateral displacement cell separation on immune cells of Marine shrimp | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Cell separation, Microfluidics, Deterministic lateral displacement, Shrimp | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Murakami, Tomoki
× Murakami, Tomoki× Koiwai, Keiichiro× Suzuki, Hiroaki |
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書誌情報 |
Sensors and Actuators B : Chemical 巻 347, p. 130587, 発行日 2021-11-15 |
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抄録 | ||||||
内容記述 | For analyzing the immune system of an organism, it is necessary to classify and separate distinct types of cells based on their characteristics. Hemocytes, immune cells of shrimp, are still difficult to classify and separate with existing technologies due to the lack of obvious morphological characteristics or type-specific markers. We adapted the deterministic lateral displacement (DLD) method as a tool to separate the hemocytes of shrimp because the previous work suggested there is a relevance between the size of hemocytes and their functions. The image and flow cytometry (FCM) analyses confirmed that this microfluidics-based technology enabled the size-based separation of cells having a broad size distribution at the buffer condition suitable for the marine species. Cells separated with this chip retained their morphological information, and qRT-PCR was successfully conducted to reveal the differences in expression levels of immune-related genes between cell populations of different sizes. The present results prove that the DLD method can be an effective tool for the analysis of non-model organisms, in which the characteristics of cells are not well established and studied. |
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内容記述 | ||||||
内容記述 | 公開日: 2023-08-13 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0925-4005 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1078104X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.snb.2021.130587 | |||||
権利 | ||||||
権利情報 | (c) 2021 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.snb.2021.130587 | |||||
情報源 | ||||||
関連識別子 | https://www.sciencedirect.com/science/article/am/pii/S0925400521011552 | |||||
関連名称 | Author's Accepted Manuscript Version/PDF (Available on: 2023-08-13) | |||||
情報源 | ||||||
関連識別子 | https://www.elsevier.com/ja-jp | |||||
関連名称 | Elsevier B.V. | |||||
出版者 | ||||||
出版者 | Elsevier B.V. |