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Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype
https://oacis.repo.nii.ac.jp/records/2557
https://oacis.repo.nii.ac.jp/records/25571a758181-c730-4650-8643-137d91b924fd
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-09-26 | |||||
タイトル | ||||||
タイトル | Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | CRISPR/Cas9, Phototactic behavior, Albino, Chub mackerel, Rotifer | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Kawamura, Wataru
× Kawamura, Wataru× Hasegawa, Niki× Yamauchi, Akihiro× Kimura, Takashi× Yahagi, Hana× Tani, Reoto× Morita, Tetsuro× Yazawa, Ryosuke× Yoshizaki, Goro |
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書誌情報 |
Aquaculture 巻 560, 号 15, p. 738490, 発行日 2022-11 |
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抄録 | ||||||
内容記述 | The albino mutation has been used as a visible genetic marker to evaluate offspring resulting from transplanting donor germ cells into recipients to produce donor-derived gametes in surrogates. Using albino mutants as either recipients or donors makes it possible to easily identify the donor-derived offspring based on body color. The chub mackerel (Scomber japonicus) is a promising recipient species for xenogeneic gamete production of commercially valuable scombrids such as bluefin tuna; however, no body-color mutants of this species have been reported. Using the CRISPR/Cas9 system, we produced F1 albino chub mackerel harboring a biallelic null mutation in the solute carrier family 45a2 (slc45a2) gene. However, albino larvae ingested significantly less rotifers than wild-type larvae when reared with a normal feeding density of rotifers, resulting in zero albino larvae survival beyond 12 days post hatch. To overcome this lethality, we developed a novel “spotlight method” of larviculture, in which positive phototaxis is used to attract both rotifers and larvae to create an ultra-high-density feeding area in a larval rearing tank. Under these conditions, albino larvae ingested as much rotifers as wild-type larvae, thus improving albino larvae survival. Finally, we confirmed albino chub mackerel completely lack melanin pigmentation, indicating that we have developed a potentially useful recipient for germ cell transplantation in Scombridae species. |
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内容記述 | ||||||
内容記述 | 公開日: 2024-06-14 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0044-8486 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00543624 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.aquaculture.2022.738490 | |||||
権利 | ||||||
権利情報 | (c) 2022 Elsevier B.V.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.aquaculture.2022.738490 | |||||
情報源 | ||||||
関連識別子 | https://www.sciencedirect.com/science/article/am/pii/S0044848622006068 | |||||
関連名称 | Author's Accepted Manuscript Version/PDF (Available on: 2024-06-14) | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20H00430/ | |||||
関連名称 | KAKEN研究課題: 代理親魚を使っておいしい魚を育種する | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000070281003/ | |||||
関連名称 | 研究代表者: 吉崎 悟朗 (東京海洋大学) | |||||
情報源 | ||||||
関連識別子 | https://www.elsevier.com/ja-jp | |||||
関連名称 | Elsevier B.V. | |||||
件名 | ||||||
主題 | 科学研究費研究成果 | |||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
科学研究費研究課題 | ||||||
主題 | 代理親魚を使っておいしい魚を育種する | |||||
科学研究費研究課題 | ||||||
主題 | Establishment of tasty fish strain using surrogate broodstock | |||||
研究課題番号 | ||||||
内容記述 | 20H00430 |