WEKO3
アイテム
{"_buckets": {"deposit": "db6a4a6d-3acf-4447-a1e6-be9ac0c214dc"}, "_deposit": {"created_by": 15, "id": "2382", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "2382"}, "status": "published"}, "_oai": {"id": "oai:oacis.repo.nii.ac.jp:00002382", "sets": ["555", "618"]}, "author_link": ["3697", "1991", "3768", "2513", "3695"], "item_3_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "32", "bibliographicPageStart": "25", "bibliographicVolumeNumber": "13", "bibliographic_titles": [{"bibliographic_title": "海洋深層水利用学会"}, {"bibliographic_title": "Deep Ocean Water Research", "bibliographic_titleLang": "en"}]}]}, "item_3_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "鹿児島湾水深218mの海底堆積物から分離された放線菌のケラチナーゼ生産の培養条件を検討し、さらに培養液から本酵素を単離・精製して諸性状を明らかにした。高い酵素活性は1/4強度(25%,v/v)の人工海水濃度で見られた。培養液上清からイオン交換及びゲル濾過クロマトグラフィーによりケラチナーゼを精製して分子量が約3万で、熱及びpH安定性の高いことを確認した。\nまた,本粗酵素はカイメン,マダイのウロコ,羽毛および羊毛などの難分解性ケラチンを原型を留めないほど分解したが、アオウミガメの甲羅およびミンククジラのヒゲなどの難分解性ケラチンはほとんど分解しなかった。", "subitem_description_type": "Abstract"}, {"subitem_description": "Cultivation conditions for keratinase production by an actinomycete strain isolated from deep-sea sediments of Kagoshima Bay at a depth of 218 m were investigated and character ized. Maximum production of the enzyme was obtained in a quarter strength artificial seawater. Purified enzyme obtained from the culture supernatant of the strain using cation exchange chromatography and gel filtration showed an approximate molecular weight of 30,000D with thermostable and pH stable characteristics. \nVarious native keratins such as sea sponge, scales of red seabream, feather and sheep wool were completely decomposed by the partially purified enzyme, whereas shell of green turtle and barbell of minke whale were hardly decomposed.", "subitem_description_type": "Abstract"}]}, "item_3_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "海洋深層水利用学会"}]}, "item_3_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.11174/dowas.13.25", "subitem_relation_type_select": "DOI"}}]}, "item_3_relation_13": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Publisher\u0027s Version/PDF (OpenAccess)"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.11174/dowas.13.25", "subitem_relation_type_select": "DOI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "海洋深層水利用学会"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.dowas.net/", "subitem_relation_type_select": "URI"}}]}, "item_3_relation_28": {"attribute_name": "論文ID(NAID)", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "130007856425", "subitem_relation_type_select": "NAID"}}]}, "item_3_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1345-8477", "subitem_source_identifier_type": "ISSN"}]}, "item_3_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11858274", "subitem_source_identifier_type": "NCID"}]}, "item_3_text_61": {"attribute_name": "タイトル(ヨミ)", "attribute_value_mlt": [{"subitem_text_value": "シンカイ タイセキブツ カラ ブンリ シタ ホウシャキン ノ セイサン スル ケラチナーゼ ト ナンブンリセイ ケラチン ノ ブンリ"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "今田, 千秋"}], "nameIdentifiers": [{"nameIdentifier": "1991", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90183011", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90183011"}]}, {"creatorNames": [{"creatorName": "武本, 裕樹"}], "nameIdentifiers": [{"nameIdentifier": "3768", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小林, 武志"}], "nameIdentifiers": [{"nameIdentifier": "2513", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60242327", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60242327"}]}, {"creatorNames": [{"creatorName": "寺原, 猛"}], "nameIdentifiers": [{"nameIdentifier": "3695", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山田, 勝久"}], "nameIdentifiers": [{"nameIdentifier": "3697", "nameIdentifierScheme": "WEKO"}]}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "深海", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ケラチナーゼ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "放線菌", "subitem_subject_scheme": "Other"}, {"subitem_subject": "難分離性ケラチン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "堆積物", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Deep-sea", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Keratinase", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Actinomycete", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Native keratin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "深海堆積物から分離した放線菌の生産するケラチナーゼと難分解性ケラチンの分解", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "深海堆積物から分離した放線菌の生産するケラチナーゼと難分解性ケラチンの分解"}, {"subitem_title": "Production of keratinase by an actinomycete strain isolated from deep-sea sediment and its decomposition capability on native keratines", "subitem_title_language": "en"}]}, "item_type_id": "3", "owner": "15", "path": ["555", "618"], "permalink_uri": "https://oacis.repo.nii.ac.jp/records/2382", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-04-07"}, "publish_date": "2022-04-07", "publish_status": "0", "recid": "2382", "relation": {}, "relation_version_is_last": true, "title": ["深海堆積物から分離した放線菌の生産するケラチナーゼと難分解性ケラチンの分解"], "weko_shared_id": -1}
深海堆積物から分離した放線菌の生産するケラチナーゼと難分解性ケラチンの分解
https://oacis.repo.nii.ac.jp/records/2382
https://oacis.repo.nii.ac.jp/records/23820bb4b701-1f7f-4230-88bf-dbe69c8f67b5
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-04-07 | |||||
タイトル | ||||||
タイトル | 深海堆積物から分離した放線菌の生産するケラチナーゼと難分解性ケラチンの分解 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Production of keratinase by an actinomycete strain isolated from deep-sea sediment and its decomposition capability on native keratines | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | 深海 | |||||
キーワード | ||||||
主題 | ケラチナーゼ | |||||
キーワード | ||||||
主題 | 放線菌 | |||||
キーワード | ||||||
主題 | 難分離性ケラチン | |||||
キーワード | ||||||
主題 | 堆積物 | |||||
キーワード | ||||||
主題 | Deep-sea | |||||
キーワード | ||||||
主題 | Keratinase | |||||
キーワード | ||||||
主題 | Actinomycete | |||||
キーワード | ||||||
主題 | Native keratin | |||||
キーワード | ||||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
タイトル(ヨミ) | ||||||
シンカイ タイセキブツ カラ ブンリ シタ ホウシャキン ノ セイサン スル ケラチナーゼ ト ナンブンリセイ ケラチン ノ ブンリ | ||||||
著者 |
今田, 千秋
× 今田, 千秋× 武本, 裕樹× 小林, 武志× 寺原, 猛× 山田, 勝久 |
|||||
書誌情報 |
海洋深層水利用学会 en : Deep Ocean Water Research 巻 13, 号 1, p. 25-32, 発行日 2012 |
|||||
抄録 | ||||||
内容記述 | 鹿児島湾水深218mの海底堆積物から分離された放線菌のケラチナーゼ生産の培養条件を検討し、さらに培養液から本酵素を単離・精製して諸性状を明らかにした。高い酵素活性は1/4強度(25%,v/v)の人工海水濃度で見られた。培養液上清からイオン交換及びゲル濾過クロマトグラフィーによりケラチナーゼを精製して分子量が約3万で、熱及びpH安定性の高いことを確認した。 また,本粗酵素はカイメン,マダイのウロコ,羽毛および羊毛などの難分解性ケラチンを原型を留めないほど分解したが、アオウミガメの甲羅およびミンククジラのヒゲなどの難分解性ケラチンはほとんど分解しなかった。 |
|||||
抄録 | ||||||
内容記述 | Cultivation conditions for keratinase production by an actinomycete strain isolated from deep-sea sediments of Kagoshima Bay at a depth of 218 m were investigated and character ized. Maximum production of the enzyme was obtained in a quarter strength artificial seawater. Purified enzyme obtained from the culture supernatant of the strain using cation exchange chromatography and gel filtration showed an approximate molecular weight of 30,000D with thermostable and pH stable characteristics. Various native keratins such as sea sponge, scales of red seabream, feather and sheep wool were completely decomposed by the partially purified enzyme, whereas shell of green turtle and barbell of minke whale were hardly decomposed. |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1345-8477 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11858274 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.11174/dowas.13.25 | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.11174/dowas.13.25 | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | http://www.dowas.net/ | |||||
関連名称 | 海洋深層水利用学会 | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 130007856425 | |||||
出版者 | ||||||
出版者 | 海洋深層水利用学会 |