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Occurrence of a metalloprotease (thermolysin) inhibitor among Brevibacillus species and purification of such inhibitor from Brevibacillus reuszeri IFO 15719^T
https://oacis.repo.nii.ac.jp/records/2354
https://oacis.repo.nii.ac.jp/records/23549ae77546-924a-458f-998d-94112e99bc7b
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-03-29 | |||||
タイトル | ||||||
タイトル | Occurrence of a metalloprotease (thermolysin) inhibitor among Brevibacillus species and purification of such inhibitor from Brevibacillus reuszeri IFO 15719^T | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Brevibacillus laterosporus, Brevibacillus reuszeri, metalloprotease, protease inhibitors, thermolysin | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Kobayashi, Takeshi
× Kobayashi, Takeshi× Nakagawa, Naoji× Imada, Chiaki× 佐藤, 奈保子 |
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書誌情報 |
Fisheries Science 巻 70, 号 2, p. 299-305, 発行日 2004-04-01 |
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抄録 | ||||||
内容記述 | The extracellular production of a thermolysin inhibitor by 534 wild-type strains isolated from marine sediments and related-type strains was examined. The inhibition of thermolysin activity by strain T-177 was observed on a casein agar medium. According to our detailed taxonomic study, strain T-177 is related to Brevibacillus laterosporus. Further screening for a thermolysin inhibitor was performed using strain T-177 and nine other taxonomically related Brevibacillus type strains, and the ability to produce the inhibitor was observed in five strains. Among these strains, B. reuszeri IFO 15719^T exhibited the highest thermolysin inhibitory activity in broth media, and thus was used for the purification and characterization of the inhibitor. Chromatographic analyses suggested that this substance is a monomeric protein with a molecular mass of 60 kDa. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0919-9268 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10993718 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1111/j.1444-2906.2003.00804.x | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1111/j.1444-2906.2003.00804.x | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | https://jsfs.jp/ | |||||
関連名称 | Japanese Society of Fisheries Science | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 10012038292 | |||||
出版者 | ||||||
出版者 | Japanese Society of Fisheries Science |