WEKO3
アイテム
ナンキョクオキアミ・カテコールオキシターゼの精製および特性
https://oacis.repo.nii.ac.jp/records/1987
https://oacis.repo.nii.ac.jp/records/19878498fafe-e384-49a7-97d1-05cc9b83aab8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2020-09-28 | |||||||||||
| タイトル | ||||||||||||
| タイトル | ナンキョクオキアミ・カテコールオキシターゼの精製および特性 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Purification and Properties of Catechol Oxidase from the Antarctic Krill | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | オキアミ | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 酵素 | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 著者 |
大島, 敏明
× 大島, 敏明
WEKO
2116
× Nagayama, Fumio |
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| 書誌情報 |
日本水産学会誌 en : Nippon Suisan Gakkaishi 巻 46, 号 8, p. 1035-1042, 発行日 1980-08 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | A catechol oxidase was isolated from the crude extract of the Antarctic krill, Euphausia superba, by a combination of ammonium sulfate treatment, ion exchange chromatography on DEAE-cellulose, and gel filtration on Sepharose 6B. The specific activity was increased about 36-fold from the supernatant of crude extract. The enzyme preparation was found to be homogeneous by polyacrylamide gel disc-electrophoresis. Optimum pH of the enzyme activity for catechol was found to be 6.5. Molecular weight of the enzyme was found to be 314, 000. One molecule was found to be composed of four subunits, and two of them had molecular weight of 75, 000 and the others 83, 000. Isoelectric point of the enzyme was pH 4.50. The activity was inhibited by potassium cyanide and sodium azide. Diethyldithiocarbamate did not inhibit the enzyme. The enzyme oxidized only a limited number of o-diphenols such as catechol and trihydroxy phenols such as pyrogallol. Monophenols such as L-tyrosine, the derivatives of L-tyrosine and L-dihydroxyphenylalanine (L-dopa) were not oxidized by the krill enzyme. In this respect, the enzyme of the krill was considered to be not tyrosinase (EC 1.14. 18.1 )but catechol oxidase (EC 1. 10. 3. 1). | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0021-5392 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN00193422 | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.2331/suisan.46.1035 | |||||||||||
| 情報源 | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.2331/suisan.46.1035 | |||||||||||
| 関連名称 | Publisher's Version/PDF (OpenAccess) | |||||||||||
| 情報源 | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://jsfs.jp/ | |||||||||||
| 関連名称 | 日本水産学会 | |||||||||||
| 論文ID（NAID） | ||||||||||||
| 識別子タイプ | NAID | |||||||||||
| 関連識別子 | 130000920632 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 日本水産学会 | |||||||||||
