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Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif
https://oacis.repo.nii.ac.jp/records/1782
https://oacis.repo.nii.ac.jp/records/1782b66f042d-34c1-4648-9d76-d9b7759d77f0
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-06-24 | |||||
タイトル | ||||||
タイトル | Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Glycobiology, Pattern recognition receptors | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Alenton, Rod Russel Reyes
× Alenton, Rod Russel Reyes× Koiwai, Keiichiro× Miyaguchi, Kohei× Kondo, Hidehiro× Hirono, Ikuo |
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書誌情報 |
Scientific Reports 巻 7, 発行日 2017-04-04 |
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抄録 | ||||||
内容記述 | C-type lectins (CTLs) are calcium-dependent carbohydrate-binding proteins known to assist the innate immune system as pattern recognition receptors (PRRs). The binding specificity of CTLs lies in the motif of their carbohydrate recognition domain (CRD), the tripeptide motifs EPN and QPD bind to mannose and galactose, respectively. However, variants of these motifs were discovered including a QAP sequence reported in shrimp believed to have the same carbohydrate specificity as QPD. Here, we characterized a novel C-type lectin (MjGCTL) possessing a CRD with a QAP motif. The recombinant MjGCTL has a calcium-dependent agglutinating capability against both Gram-negative and Gram-positive bacteria, and its sugar specificity did not involve either mannose or galactose. In an encapsulation assay, agarose beads coated with rMjGCTL were immediately encapsulated from 0 h followed by melanization at 4 h post-incubation with hemocytes. These results confirm that MjGCTL functions as a classical CTL. The structure of QAP motif and carbohydrate-specificity of rMjGCTL was found to be different to both EPN and QPD, suggesting that QAP is a new motif. Furthermore, MjGCTL acts as a PRR binding to hemocytes to activate their adherent state and initiate encapsulation. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 28374848 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1038/srep45818 | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1038/srep45818 | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-15H02462/ | |||||
関連名称 | KAKEN研究課題: 病原微生物の侵入・攻撃に対するクルマエビ類の免疫応答に関する研究 | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000000270926/ | |||||
関連名称 | 研究代表者: 廣野 育生 (東京海洋大学) | |||||
件名 | ||||||
主題 | 科学研究費研究成果 | |||||
出版者 | ||||||
出版者 | Springer Nature Publishing AG | |||||
科学研究費研究課題 | ||||||
主題 | 病原微生物の侵入・攻撃に対するクルマエビ類の免疫応答に関する研究 | |||||
科学研究費研究課題 | ||||||
主題 | Immune response of kuruma shrimp against microbial infections | |||||
研究課題番号 | ||||||
内容記述 | 15H02462 |