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Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: characterization of vasa, dead end, and lymphocyte antigen 75 genes
https://oacis.repo.nii.ac.jp/records/1635
https://oacis.repo.nii.ac.jp/records/16350ad34f53-f015-4373-8ae4-238cdac2b61b
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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公開日 | 2019-01-07 | |||||||||||||||
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タイトル | Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: characterization of vasa, dead end, and lymphocyte antigen 75 genes | |||||||||||||||
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言語 | eng | |||||||||||||||
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主題 | germ cell marker, germ cell migration, germ cell specification, PGC development | |||||||||||||||
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資源タイプ | journal article | |||||||||||||||
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アクセス権 | metadata only access | |||||||||||||||
著者 |
Kazue Nagasawa
× Kazue Nagasawa
× Jorge M.O. Fernandes
× Yoshizaki, Goro
× Misako Miwa
× Igor Babiak
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書誌情報 |
Molecular Reproduction and Development 巻 80, 号 2, p. 118-131, 発行日 2012-12-23 |
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内容記述 | No information exists on the identification of primordial germ cells (PGCs) in superorder Protacanthopterygii, which includes the Salmonidae family and Atlantic salmon (Salmo salar L.), one of the most commercially important aquatic animals worldwide. In order to identify salmon PGCs, we cloned the full-length cDNA of vasa, dead end (dnd), and lymphocyte antigen 75 (ly75/CD205) genes as germ cell marker candidates, and analyzed their expression patterns in both adult and embryonic stages of Atlantic salmon. Semi-quantitative RT-PCR results showed that salmon vasa and dnd were specifically expressed in testis and ovary, and vasa, dnd, and ly75 mRNA were maternally deposited in the egg. Vasa mRNA was consistently detected throughout embryogenesis, while dnd and ly75 mRNA were gradually degraded during cleavages. Further in situ hybridization (ISH) analyses revealed the localization of vasa, dnd mRNA, and Ly75 protein in PGCs of hatched larvae. Whole-mount ISH (WISH) detected vasa mRNA during embryogenesis, showing a distribution pattern somewhat different to that of zebrafish; specifically, at mid-blastula stage, vasa-expressing cells were randomly distributed at the central part of blastodisc, and then they migrated to the presumptive region of embryonic shield. Therefore, the typical vasa localization pattern of four clusters during blastulation, as found in zebrafish, was not present in Atlantic salmon. In addition, salmon PGCs could be specifically labeled with a green fluorescence protein (GFP) using Gfp-rt-vasa 3’UTR RNA microinjection for further applications. These findings may assist in understanding PGC development not only in Atlantic salmon but also in other salmonids. | |||||||||||||||
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収録物識別子タイプ | ISSN | |||||||||||||||
収録物識別子 | 1040452X | |||||||||||||||
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収録物識別子タイプ | NCID | |||||||||||||||
収録物識別子 | AA10735246 | |||||||||||||||
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DOI | ||||||||||||||||
識別子タイプ | DOI | |||||||||||||||
関連識別子 | 10.1002/mrd.22142 | |||||||||||||||
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関連識別子 | https://doi.org/10.1002/mrd.22142 | |||||||||||||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||||||||||||
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関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-25114005/ | |||||||||||||||
関連名称 | KAKEN研究課題: サケ科魚類の進化に伴うGSC制御機構の変化 | |||||||||||||||
情報源 | ||||||||||||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000070281003/ | |||||||||||||||
関連名称 | 研究代表者: 吉崎 悟朗 (東京海洋大学) | |||||||||||||||
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主題 | 科学研究費研究成果 | |||||||||||||||
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出版者 | Wiley | |||||||||||||||
科学研究費研究課題 | ||||||||||||||||
主題 | サケ科魚類の進化に伴うGSC制御機構の変化 | |||||||||||||||
科学研究費研究課題 | ||||||||||||||||
主題 | Evolution of GSC regulation in salmonids Planned | |||||||||||||||
研究課題番号 | ||||||||||||||||
内容記述 | 25114005 |