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Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method
https://oacis.repo.nii.ac.jp/records/2355
https://oacis.repo.nii.ac.jp/records/23551ca64f3f-b6b6-470d-98f0-17c062393e0c
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-03-29 | |||||
タイトル | ||||||
タイトル | Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | fusion, protoplast, recombinant, regeneration, Streptomyces | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Imada, Chiaki
× Imada, Chiaki× Ikemoto, Yuko× Kobayashi, Takeshi× Hamada, Naoko× Watanabe, Etsuo |
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書誌情報 |
Fisheries Science 巻 68, 号 2, p. 395-402, 発行日 2002-04 |
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抄録 | ||||||
内容記述 | Protoplast fusion between different species of Streptomyces was performed using a liquid regeneration method developed for a rapid and simple preparation of the fusants. Consequently, new clones, which could not be obtained using the conventional agar regeneration method, were obtained. In the crosses between S. griseus and S. durhamensis, and between S. californicus and S. catenulae, eight and two recombinants, respectively, were obtained using the liquid regeneration method. Conversely, in the case of crosses between S. ornatus and S. catenulae, and between S. ornatus and S. vendargensis, seven recombinants each were obtained using only the agar method. The physiological characteristics, such as the assimilation of carbohydrate and antibiotic resistance, of these fusants differed considerably from those of their parental strains. Using the proposed liquid regeneration method, a simpler and quicker procedure for protoplast fusion is described. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0919-9268 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10993718 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1046/j.1444-2906.2002.00438.x | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1046/j.1444-2906.2002.00438.x | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | https://jsfs.jp/ | |||||
関連名称 | 日本水産学会 | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 10008273357 | |||||
出版者 | ||||||
出版者 | 日本水産学会 |