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アイテム
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Surrogate Production of Eggs and Sperm by Intrapapillary Transplantation of Germ Cells in Cytoablated Adult Fish
https://oacis.repo.nii.ac.jp/records/1800
https://oacis.repo.nii.ac.jp/records/1800e1274936-830e-4f20-b3a1-9044850840af
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-07-08 | |||||
タイトル | ||||||
タイトル | Surrogate Production of Eggs and Sperm by Intrapapillary Transplantation of Germ Cells in Cytoablated Adult Fish | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Gonads, Germ cells, Histology, Sperm, Oogonia, Cell transplantation, Polymerase chain reaction, Spermatogonia | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Majhi, Sullip Kumar
× Majhi, Sullip Kumar× Hattori, Ricardo Shohei× Rahman, Sheikh Mustafizur× Strüssmann, Carlos Augusto |
|||||
書誌情報 |
PLoS ONE 巻 9, 号 4, p. e95294, 発行日 2014-04-18 |
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抄録 | ||||||
内容記述 | Germ cell transplantation (GCT) is a promising assisted reproductive technology for the conservation and propagation of endangered and valuable genetic resources. In teleost fish, GCT in adult gonads has been achieved only in male recipients, limiting greatly the usefulness of this technique in situations where both sexes need equal and timely attention for conservation and/or propagation. Here we describe a simplified GCT approach that ultimately leads to production of donor-derived eggs and sperm in considerably short time. Donor germ cells isolated from young pejerrey Odontesthes bonariensis (Atherinopsidae) were transplanted non-surgically through the genital papilla into the sexually mature gonads of Patagonian pejerrey O. hatcheri recipients whose gonads have been depleted of endogenous GCs by heat (26°C) and chemical treatment (four doses of Busulfan at 30 mg/kg and 40 mg/kg for females and males, respectively). Transplanted spermatogonial and oogonial cells were able to recolonize the recipients' gonads and produce functional donor origin eggs and sperm within 7 months from the GCT. We confirmed the presence of donor-derived gametes by PCR in 17% and 5% of the surrogate O. hatcheri fathers and mothers, respectively. The crosses between surrogate fathers and O. bonariensis mothers yielded 12.6–39.7% pure O. bonariensis and that between a surrogate mother and an O. bonariensis father yielded 52.2% pure O. bonariensis offspring. Our findings confirm that transplantation of germ cells into sexually competent adult fish by non-surgical methods allows the production of functional donor-derived eggs and sperm in a considerably short time. The methods described here could play a vital role in conservation and rapid propagation of endangered fish genetic resources. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1932-6203 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1371/journal.pone.0095294 | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1371/journal.pone.0095294 | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-26241018/ | |||||
関連名称 | KAKEN研究課題: 魚類繁殖機構に及ぼす地球温暖化・気候変動影響の早期警戒指標の構築に向けて | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000010231052/ | |||||
関連名称 | 研究代表者: ストルスマン C.A. (東京海洋大学) | |||||
件名 | ||||||
主題 | 科学研究費研究成果 | |||||
出版者 | ||||||
出版者 | PLOS | |||||
科学研究費研究課題 | ||||||
主題 | 魚類繁殖機構に及ぼす地球温暖化・気候変動影響の早期警戒指標の構築に向けて | |||||
科学研究費研究課題 | ||||||
主題 | Towards an early warning information system for the effects of global warming/climate change on fish reproduction | |||||
研究課題番号 | ||||||
内容記述 | 26241018 |