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Characterization of abnZ2 (yxiA1) and abnZ3 (yxiA3) in Paenibacillus polymyxa, encoding two novel endo-1,5-α-l-arabinanases
https://oacis.repo.nii.ac.jp/records/1787
https://oacis.repo.nii.ac.jp/records/17877141f5e5-4f8f-494f-ac47-34ea8076ea53
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-06-24 | |||||
タイトル | ||||||
タイトル | Characterization of abnZ2 (yxiA1) and abnZ3 (yxiA3) in Paenibacillus polymyxa, encoding two novel endo-1,5-α-l-arabinanases | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Paenibacillus polymyxa Z6, Endo-arabinanase, Cold-adapted enzyme | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
著者 |
Wang, Shaohua
× Wang, Shaohua× Yang, Ying× Zhang, Jian× Sun, Jiaqi× Matsukawa, Shingo× Xie, Jingli× Wei, Dongzhi |
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書誌情報 |
Bioresources and Bioprocessing 巻 1, 号 14, 発行日 2014-09-24 |
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抄録 | ||||||
内容記述 | Background: Protopectinases which were consisted of various different enzymes can promote the solubilization of protopectin from the plant cell and can be applied in the protein industry extraction. The genome sequence of Paenibacillus polymyxa Z6 that produces a protopectinases complex was partially determined. Two new genes, yxiA1 and yxiA3, were identified as uncharacterized protein in the P. polymyxa genome. And, they were classified as the member of the glycoside hydrolase family 43 (GH43) according to the primary protein sequence. | |||||
抄録 | ||||||
内容記述 | Results: The two genes were cloned and expressed in Escherichia coli BL21 (DE3). And, the results indicated that the product of yxiA1 and yxiA3 were two endo-α-1,5-l-arabinanases. Thus, the two genes were renamed as abnZ2 (yxiA1) and abnZ3 (yxiA3). Recombinant AbnZ2 had optimal activity at pH 6.0 and 35°C. And, AbnZ3 had optimal activity at pH 6.0 and 30°C. However, unlike most reported endo-arabinanases, the specific activity of AbnZ3 remained 48.7% of maximum at 5°C, which meant AbnZ3 was an excellent cold-adapted enzyme. | |||||
抄録 | ||||||
内容記述 | Conclusions: This paper demonstrated that the gene yxiA1 and yxiA3 were two new endo-arabinanases, and renamed as abnZ2 and abnZ3. Moreover AbnZ3 was an excellent cold-adapted enzyme which could be attractive in fruit juice processing. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2197-4365 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1186/s40643-014-0014-8 | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1186/s40643-014-0014-8 | |||||
関連名称 | Publisher's Version/PDF (OpenAccess) | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-26282017/ | |||||
関連名称 | KAKEN研究課題: 食品ハイドロゲルのミクロ相分離構造がテクスチャーに与える影響 | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000030293096/ | |||||
関連名称 | 研究代表者: 松川 真吾 (東京海洋大学) | |||||
件名 | ||||||
主題 | 科学研究費研究成果 | |||||
出版者 | ||||||
出版者 | Springer Nature | |||||
科学研究費研究課題 | ||||||
主題 | 食品ハイドロゲルのミクロ相分離構造がテクスチャーに与える影響 | |||||
科学研究費研究課題 | ||||||
主題 | Effect of Micro Phase Separation in Food Hydrogels on Texture | |||||
研究課題番号 | ||||||
内容記述 | 26282017 |